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Objective <p>The global rise of antimicrobial resistance with the spread of pathogenic multidrug-resistant clones such as <i>Escherichia coli</i> sequence type (ST) 131 represents a major challenge for public health, renewing interest in bacteriophages as therapeutical agents.</p> Results <p>We report the isolation and characterization of a new bacteriophage, <i>Escherichia</i> phage vB_EcoM-V1EC45, which was isolated from wastewater and was able to infect a clinical <i>E. coli</i> ST131 strain. Its genome is a 170,364&#xa0;bp double-stranded DNA molecule annotated with 281 coding sequences and 9 tRNAs, including multiple genes involved in antidefense systems. V1EC45 is predicted to harbour a virulent lifestyle. Comparative genomics positioned V1EC45 within the <i>Tequatrovirus</i> genus, and the Tsx outer membrane bacterial protein is predicted to be used as the receptor-binding protein. This work highlights the value of using genomic, structural, and evolutionary analyses to support the directed development of targeted bacteriophage therapeutics.</p>

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Genomic characterization and comparative analysis of a new Tequatrovirus phage infecting Escherichia coli ST131

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年5月16日

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  • Elsa Beurrier,
  • Floriane Laumay,
  • Juliette Hayer,
  • Jacques Dainat,
  • Mélanie Bonhomme,
  • Mathieu Medina,
  • Camille Kolenda,
  • Richard Bonnet,
  • Laurent Dortet,
  • Frédéric Laurent,
  • Rémy Froissart

摘要

Objective

The global rise of antimicrobial resistance with the spread of pathogenic multidrug-resistant clones such as Escherichia coli sequence type (ST) 131 represents a major challenge for public health, renewing interest in bacteriophages as therapeutical agents.

Results

We report the isolation and characterization of a new bacteriophage, Escherichia phage vB_EcoM-V1EC45, which was isolated from wastewater and was able to infect a clinical E. coli ST131 strain. Its genome is a 170,364 bp double-stranded DNA molecule annotated with 281 coding sequences and 9 tRNAs, including multiple genes involved in antidefense systems. V1EC45 is predicted to harbour a virulent lifestyle. Comparative genomics positioned V1EC45 within the Tequatrovirus genus, and the Tsx outer membrane bacterial protein is predicted to be used as the receptor-binding protein. This work highlights the value of using genomic, structural, and evolutionary analyses to support the directed development of targeted bacteriophage therapeutics.