Objectives <p>Breast cancer is a complex malignancy influenced by both genetic and environmental mechanisms. The WDFY4 gene, predominantly expressed in immune tissues, plays a role in immune regulation. However, its application in relation to breast cancer risk among the Bangladeshi women has not been explored. The proposed case-control study was designed to determine the relationship between the susceptibility to breast cancer and the two WDFY4 polymorphisms, rs11101565 and rs2671702 and the association was evaluated by the T-ARMS-PCR method.</p> Results <p>For rs11101565, the CA heterozygous genotype conferred a 1.36-fold increased risk compared to the CC wild-type genotype, though this association did not reach statistical significance (<i>p</i> &gt; 0.05). Dominant and recessive models suggested elevated risk among AA homozygotes, while the overdominant model revealed a modest correlation; however, none achieved statistical significance. For rs2671702, the GA heterozygous genotype was associated with increased risk relative to GG, whereas the AA genotype exhibited a protective trend, yet all associations remained non-significant. Inconsistent risk patterns were observed across dominant and recessive models, while the minor allele A and overdominant model demonstrated stronger but statistically non-significant associations. Collectively, these findings indicate that WDFY4 polymorphisms (rs11101565 and rs2671702) do not significantly influence breast cancer susceptibility among Bangladeshi women.</p>