Scholarlink

Objective <p>Anchorage-independent growth is a critical feature of cancer cells, reflecting their ability to survive and proliferate without attachment to the extracellular matrix. Spheres—cancerous masses formed in a three-dimensional (3D) anchorage-independent culture—contain a high level of cancer stem cells. This anchorage-independent proliferative capacity closely relates to tumorigenicity, anoikis resistance, and metastatic capability. Pancreatic ductal adenocarcinoma (PDAC) is a heterogeneous group comprising epithelial and mesenchymal features, and these subtypes exhibit different biological characteristics in 3D cultures. This study examines whether these PDAC subtypes differ in their anchorage-independent proliferative capability at the single-cell level.</p> Methods <p>Eight PDAC cell lines, including five epithelial-type and three mesenchymal-type lines, were cultured as single cells in poly (2-methacryloyloxyethyl phosphorylcholine) (MPC) polymer–coated low-attachment microwell plates, and time-lapse imaging was performed every 15&#xa0;min for 60&#xa0;h.</p> Results <p>Three phenotypes were observed: non-proliferating single cells, cells dividing into two, and those forming clusters of three or four cells. In single-cell analysis, KP4 and MIA PaCa-2 mesenchymal PDAC cells exhibited a high number of cells proliferating into two or more cells.</p> Conclusion <p>These findings suggest that mesenchymal PDAC cells exhibit greater anchorage-independent proliferative capability, reflecting their aggressive biological behavior.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Single-cell analysis of anchorage-independent growth ability in pancreatic ductal adenocarcinoma cell lines

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年1月24日

查看全文
  • Yuuki Shichi,
  • Seiichi Shinji,
  • Masakazu Fujiwara,
  • Yutaro Ogawa,
  • Yusuke Yoshimura,
  • Keisuke Nonaka,
  • Hiroshi Yoshida,
  • Toshiyuki Ishiwata

摘要

Objective

Anchorage-independent growth is a critical feature of cancer cells, reflecting their ability to survive and proliferate without attachment to the extracellular matrix. Spheres—cancerous masses formed in a three-dimensional (3D) anchorage-independent culture—contain a high level of cancer stem cells. This anchorage-independent proliferative capacity closely relates to tumorigenicity, anoikis resistance, and metastatic capability. Pancreatic ductal adenocarcinoma (PDAC) is a heterogeneous group comprising epithelial and mesenchymal features, and these subtypes exhibit different biological characteristics in 3D cultures. This study examines whether these PDAC subtypes differ in their anchorage-independent proliferative capability at the single-cell level.

Methods

Eight PDAC cell lines, including five epithelial-type and three mesenchymal-type lines, were cultured as single cells in poly (2-methacryloyloxyethyl phosphorylcholine) (MPC) polymer–coated low-attachment microwell plates, and time-lapse imaging was performed every 15 min for 60 h.

Results

Three phenotypes were observed: non-proliferating single cells, cells dividing into two, and those forming clusters of three or four cells. In single-cell analysis, KP4 and MIA PaCa-2 mesenchymal PDAC cells exhibited a high number of cells proliferating into two or more cells.

Conclusion

These findings suggest that mesenchymal PDAC cells exhibit greater anchorage-independent proliferative capability, reflecting their aggressive biological behavior.