Scholarlink

Objective <p>This study aimed to identify novel isoforms of mouse succinate dehydrogenase complex flavoprotein subunit A (Sdha) arising from internal exon skipping, analogous to the process observed in human ortholog SDHA.</p> Results <p>We identified a novel isoform, designated Δ3-10, which lacked the final 104 nucleotides of exon 3 and all of exons 4 through 10, yet did not alter the reading frame. The Δ3-10 Sdha cDNA was cloned into expression vectors, and overexpression resulted in a protein localized to the mitochondria. However, the endogenous Δ3-10 Sdha protein was not detected with the available antibodies.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Identification of a novel transcript of mouse Sdha

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年2月25日

查看全文
  • María J. Esteban-Amo,
  • Amaia Telleria,
  • Dino Gobelli,
  • Pablo Serrano-Lorenzo,
  • Juan J. Tellería,
  • María T. Pérez-García,
  • Piotr Kozlowski,
  • Miguel Á. de la Fuente,
  • María Simarro

摘要

Objective

This study aimed to identify novel isoforms of mouse succinate dehydrogenase complex flavoprotein subunit A (Sdha) arising from internal exon skipping, analogous to the process observed in human ortholog SDHA.

Results

We identified a novel isoform, designated Δ3-10, which lacked the final 104 nucleotides of exon 3 and all of exons 4 through 10, yet did not alter the reading frame. The Δ3-10 Sdha cDNA was cloned into expression vectors, and overexpression resulted in a protein localized to the mitochondria. However, the endogenous Δ3-10 Sdha protein was not detected with the available antibodies.