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Objective <p>Yeast cell-free extracts and supernatants contain several compounds such as β-glucan, mannan, chitin, and mannoprotein with potent antitumor and other health-promoting activities. Candida albicans have been frequently and widely isolated from different habitats compared to other yeasts. The supernatant extracted from this yeast also contains β-glucan, chitin, and mannan compounds. This study investigates the anticancer, apoptosis-inducing, and downregulation of proinflammatory gene expression activities in normal and drug-resistant human stomach cancer cells (EPG and RDB cell lines) after 24 and 48&#xa0;h treatment.</p> Results <p>We found that <i>Candida albicans</i> supernatant-induced apoptosis suppressed the <i>survivin</i> gene expression in both cell lines and suppressed the expression of <i>IL-8</i> and <i>NF-</i>ƙ<i>B</i> genes in normal stomach cancer cells. IC<sub>50</sub> for EPG cells were 1599&#xa0;µg/mL and 1040&#xa0;µg/mL after 24 and 48&#xa0;h treatment, respectively; and for RDB cells were 877&#xa0;µg/mL and 675&#xa0;µg/mL after 24 and 48&#xa0;h treatment, respectively. Consequently, this work suggests that <i>Candida albicans</i> supernatant can potentially protect against and treat human stomach cancer.</p>

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Candida albicans cell-free extract against human gastric cancer; an in-vitro study

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年2月27日

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  • Babak Pakbin,
  • Shaghayegh Pishkhan Dibazar,
  • Samaneh Allahyari,
  • Faezeh Mohammadi,
  • Reza Ovissipour,
  • Amir Peymani,
  • Arian Amirvaresi

摘要

Objective

Yeast cell-free extracts and supernatants contain several compounds such as β-glucan, mannan, chitin, and mannoprotein with potent antitumor and other health-promoting activities. Candida albicans have been frequently and widely isolated from different habitats compared to other yeasts. The supernatant extracted from this yeast also contains β-glucan, chitin, and mannan compounds. This study investigates the anticancer, apoptosis-inducing, and downregulation of proinflammatory gene expression activities in normal and drug-resistant human stomach cancer cells (EPG and RDB cell lines) after 24 and 48 h treatment.

Results

We found that Candida albicans supernatant-induced apoptosis suppressed the survivin gene expression in both cell lines and suppressed the expression of IL-8 and NF-ƙB genes in normal stomach cancer cells. IC50 for EPG cells were 1599 µg/mL and 1040 µg/mL after 24 and 48 h treatment, respectively; and for RDB cells were 877 µg/mL and 675 µg/mL after 24 and 48 h treatment, respectively. Consequently, this work suggests that Candida albicans supernatant can potentially protect against and treat human stomach cancer.