The impact of the physicochemical properties of calcium phosphate ceramics on biocompatibility and osteogenic differentiation of mesenchymal stem cells

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2024年10月7日

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Yuliia Skliarenko,
Volodymyr V. Kolomiiets,
Volodymyr V. Balatskyi,
Yuliia Galuza,
Oksana S. Koryak,
Larysa L. Macewicz,
Tetiana P. Ruban,
Sergey A. Firstov,
Nataliia. V. Ulianchych,
Oksana O. Piven

摘要

Objective

In this study we have focused on biocompatibility and osteoinductive capacity analysis of self-manufactured single-phase (HAP) and two-phase (HAP and β-ТСР) bioactive ceramics with various chemical modifications (Fig. 1).

Results

We demonstrate a reduction in solubility for all analyzed composite after the treatment with H₂O and H₂O₂, accompanied by an enhancement in adsorption activity. This modification also resulted in an increase in micro- and macroporosity, along with a rise in the open porosity. Adipose-derived mesenchymal stromal cells demonstrated excellent cell adhesion and survival when cultured with these ceramics. Calcium phosphate ceramics (H-500, HT-500, and HT-1 series) stimulated alkaline phosphatase expression, promoted calcium deposition, and enhanced osteopontin expression in ADSCs, independently inducing osteogenesis without additional osteogenic stimuli. These findings underscore the promising potential of HAP-based bioceramics for bone regeneration/reconstruction.

Graphical Abstract