Scholarlink

Background <p>Repetitive elements, particularly satellite DNA (satDNA), play a significant role in genome evolution and organisation. However, their diversity and evolutionary dynamics remain poorly understood in non-model organisms. Freshwater crayfish (Decapoda, Astacidea) have large genomes with a high chromosome number and are rich in satDNAs. This makes them attractive for studying the impact of satDNA on genome evolution.</p> Results <p>In this study, we investigated the repetitive genomic landscape of 19 species representing four freshwater crayfish families. Our analysis revealed a high proportion of repetitive DNA in all studied species, with the total repeat content ranging from 30% to 66%. The number of satDNA families was remarkably high (54–622 families per species), with minisatellites (&lt; 100&#xa0;bp) forming the largest component of the satellitome. Family-specific patterns emerged: Astacidae and Cambaroididae showed the highest satDNA proportions, while Cambaridae and Parastacidae were dominated by Class I transposable elements. Species of the family Parastacidae showed the largest number of unique satDNA clusters and were clearly separated from other families, reflecting their phylogenetic divergence and distinct biogeographic history. We identified specific satDNAs conserved across all species, among them the PlSAT3-411, pointing to their important functional roles as pericentromeric satDNA.</p> Conclusion <p>This study provides the first comprehensive comparative analysis of satDNA in freshwater crayfish. Our results highlight the dynamic nature of repetitive DNA and underscore its importance in genome organisation and evolutionary history.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

The extraordinary satellitome diversity of freshwater crayfish: a driver of genome evolution

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年4月28日

查看全文
  • Lena Bonassin,
  • Ljudevit Luka Boštjančić,
  • Christelle Rutz,
  • Caterina Francesconi,
  • Leonie Schardt,
  • Damian Baranski,
  • Carola Greve,
  • Lucian Pârvulescu,
  • Višnja Besendorfer,
  • Jelena Mlinarec,
  • Ivana Maguire,
  • Kathrin Theissinger,
  • Odile Lecompte

摘要

Background

Repetitive elements, particularly satellite DNA (satDNA), play a significant role in genome evolution and organisation. However, their diversity and evolutionary dynamics remain poorly understood in non-model organisms. Freshwater crayfish (Decapoda, Astacidea) have large genomes with a high chromosome number and are rich in satDNAs. This makes them attractive for studying the impact of satDNA on genome evolution.

Results

In this study, we investigated the repetitive genomic landscape of 19 species representing four freshwater crayfish families. Our analysis revealed a high proportion of repetitive DNA in all studied species, with the total repeat content ranging from 30% to 66%. The number of satDNA families was remarkably high (54–622 families per species), with minisatellites (< 100 bp) forming the largest component of the satellitome. Family-specific patterns emerged: Astacidae and Cambaroididae showed the highest satDNA proportions, while Cambaridae and Parastacidae were dominated by Class I transposable elements. Species of the family Parastacidae showed the largest number of unique satDNA clusters and were clearly separated from other families, reflecting their phylogenetic divergence and distinct biogeographic history. We identified specific satDNAs conserved across all species, among them the PlSAT3-411, pointing to their important functional roles as pericentromeric satDNA.

Conclusion

This study provides the first comprehensive comparative analysis of satDNA in freshwater crayfish. Our results highlight the dynamic nature of repetitive DNA and underscore its importance in genome organisation and evolutionary history.