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<p>Microbial cell-free DNA (mcfDNA) enables rapid and sensitive pathogen detection via direct plasma-sequencing. Here, we present a fast, cost-effective mcfDNA multiplex workflow to complement or improve the sensitivity of microbiological diagnostics for systemic and focal infections which can be challenged by prior antibiotic treatment or non-culturable pathogens. In an explorative study of 18 patients, mcfDNA sequencing matched the clinical diagnosis in 16 cases (89%), was detected up to 16&#xa0;days after starting targeted antibiotic therapy and identified <i>Staphylococcus aureus</i> in two cases of culture-negative endocarditis. By providing an open-access protocol, we aim to promote broader accessibility and globally available application.</p>

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Microbial cell-free DNA for rapid pathogen identification in clinical diagnostics: a proof of concept study

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年6月19日

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  • Micha Banz,
  • Stefan Hagel,
  • Sebastian Freiburger,
  • Frederik Huhn,
  • Merlin Krause,
  • Stefan Glöckner,
  • Bettina Löffler,
  • Dennis Nurjadi,
  • Uthayakumar Muthukumarasamy,
  • Mateusz Jundzill,
  • Achim J. Kaasch,
  • Mathias W. Pletz,
  • Christian Brandt

摘要

Microbial cell-free DNA (mcfDNA) enables rapid and sensitive pathogen detection via direct plasma-sequencing. Here, we present a fast, cost-effective mcfDNA multiplex workflow to complement or improve the sensitivity of microbiological diagnostics for systemic and focal infections which can be challenged by prior antibiotic treatment or non-culturable pathogens. In an explorative study of 18 patients, mcfDNA sequencing matched the clinical diagnosis in 16 cases (89%), was detected up to 16 days after starting targeted antibiotic therapy and identified Staphylococcus aureus in two cases of culture-negative endocarditis. By providing an open-access protocol, we aim to promote broader accessibility and globally available application.