Scholarlink

Background <p><i>Entamoeba histolytica</i> is a pathogenic protozoan accountable for amoebiasis, while <i>Entamoeba moshkovskii</i> is considered non-invasive. Despite morphological similarity, the molecular mechanisms underlying their different pathogenicity remain largely undefined.</p> Methods <p>Trophozoite proteins from axenic cultures of <i>E. histolytica</i> and <i>E. moshkovskii</i> were separated and identified using GeLC–MS/MS, and classified using Gene Ontology. Selected and differentially expressed proteins were validated by peptide-specific antibody production, ELISA, and immunofluorescence to determine cellular localization.</p> Results and discussion <p>A total of 1,077 and 1,201 proteins were identified from <i>E. histolytica</i> and <i>E. moshkovskii</i>, respectively. The 801 of <i>Entamoeba</i> common proteins included kinases, GTPase-activating proteins, and heat shock proteins, reflecting conserved cellular processes. <i>E. histolytica</i>-unique proteins involved in nitrogen compound metabolism, vesicle-mediated transport, and catalytic activities, whereas <i>E. moshkovskii</i> proteins were related to lipid metabolism and environmental resilience. Subcellular localization revealed species-specific distribution of MmpL and AIG1-family proteins, suggesting potential roles in pathogenicity and host-immune response. A large proportion of hypothetical proteins was identified, highlighting gaps and opportunities for future study.</p> Conclusions <p>Our study highlights conserved and divergent functions and cellular locations of <i>Entamoeba</i> species-specific proteins as insights for distinct pathogenicity and adaptation. MmpL and AIG1 proteins were proposed as potential targets for further diagnostic and therapeutic development.</p> Graphical Abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Comparative proteomics reveals distinct functions and localization of invasive Entamoeba histolytica and non-invasive Entamoeba moshkovskii proteins

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年6月24日

查看全文
  • Somphob Leetachewa,
  • Narumol Khomkhum,
  • Sittiruk Roytrakul,
  • Onrapak Reamtong,
  • Porntip Chavalitshewinkoon-Petmitr,
  • Saengduen Moonsom

摘要

Background

Entamoeba histolytica is a pathogenic protozoan accountable for amoebiasis, while Entamoeba moshkovskii is considered non-invasive. Despite morphological similarity, the molecular mechanisms underlying their different pathogenicity remain largely undefined.

Methods

Trophozoite proteins from axenic cultures of E. histolytica and E. moshkovskii were separated and identified using GeLC–MS/MS, and classified using Gene Ontology. Selected and differentially expressed proteins were validated by peptide-specific antibody production, ELISA, and immunofluorescence to determine cellular localization.

Results and discussion

A total of 1,077 and 1,201 proteins were identified from E. histolytica and E. moshkovskii, respectively. The 801 of Entamoeba common proteins included kinases, GTPase-activating proteins, and heat shock proteins, reflecting conserved cellular processes. E. histolytica-unique proteins involved in nitrogen compound metabolism, vesicle-mediated transport, and catalytic activities, whereas E. moshkovskii proteins were related to lipid metabolism and environmental resilience. Subcellular localization revealed species-specific distribution of MmpL and AIG1-family proteins, suggesting potential roles in pathogenicity and host-immune response. A large proportion of hypothetical proteins was identified, highlighting gaps and opportunities for future study.

Conclusions

Our study highlights conserved and divergent functions and cellular locations of Entamoeba species-specific proteins as insights for distinct pathogenicity and adaptation. MmpL and AIG1 proteins were proposed as potential targets for further diagnostic and therapeutic development.

Graphical Abstract