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Background <p><i>Eimeria necatrix</i>, a member of the Apicomplexa phylum, is one of the most pathogenic parasites, causing high mortality in chickens. Microneme proteins (MICs) play essential roles in host cell recognition and invasion by apicomplexan parasites and are also attractive candidates for vaccine development. However, comprehensive studies on <i>E. necatrix</i> MICs remain limited.</p> Methods <p><i>Eimeria necatrix MIC3</i> gene (En<i>MIC3</i>) was amplified and expressed in <i>Escherichia coli</i>. The recombinant protein (rEnMIC3) was characterized via SDS-PAGE and Western blot. The antigenicity of rEnMIC3 and its localization in sporozoites (SZ) and second-generation merozoites (MZ-2) of <i>E. necatrix</i> were determined by Western blot and indirect immunofluorescence analyses (IFAs). The dynamic expression of EnMIC3 across different developmental stages and its impact on sporozoite invasion of host cells were analyzed. The immune protection provided by rEnMIC3 was evaluated in chickens using weight gain, lesion scores, oocyst production, anticoccidial index (ACI), and antibody levels.</p> Results <p>The open reading frame of En<i>MIC3</i> was 798&#xa0;bp, encoding a 265-amino acid protein with a predicted molecular weight of 28.50&#xa0;kDa. EnMIC3 contained a signal peptide and a single epidermal growth factor (EGF)-like domain. The rEnMIC3 with an approximate molecular weight of 36&#xa0;kDa could be specifically recognized by convalescent sera from chickens infected with <i>E. necatrix</i>. The molecular mass of the native protein was approximately 35&#xa0;kDa, and it localizes to the apical region in SZ but exhibits a cytoplasmic distribution in MZ-2. EnMIC3 mRNA was expressed at significantly higher levels in SZ than in MZ-2, whereas protein expression displayed an inverse pattern. Anti-rEnMIC3 polyclonal antibodies inhibited sporozoite invasion of DF-1 cells in a dose-dependent manner. Vaccination with rEnMIC3 conferred effective protection against <i>E. necatrix</i> challenge, with the high-dose group (200&#xa0;µg) achieving the highest ACI value (171.32) and markedly elevated serum antibody levels.</p> Conclusions <p>These findings not only offer a foundation for understanding the role of EnMIC3 protein in the host invasion of <i>E. necatrix</i> but also present a potential protective antigen of <i>E. necatrix</i> for the development of a subunit vaccine against avian coccidiosis.</p> Graphical Abstract <p></p>

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Molecular characterization and immunoprotective potential of microneme protein 3 from Eimeria necatrix

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年5月2日

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  • Qianqian Feng,
  • Danli Yan,
  • Nianyu Xue,
  • Dandan Liu,
  • Weimin Cai,
  • Yuxin Zhou,
  • Zhaofeng Hou,
  • Jinjun Xu,
  • Jianping Tao

摘要

Background

Eimeria necatrix, a member of the Apicomplexa phylum, is one of the most pathogenic parasites, causing high mortality in chickens. Microneme proteins (MICs) play essential roles in host cell recognition and invasion by apicomplexan parasites and are also attractive candidates for vaccine development. However, comprehensive studies on E. necatrix MICs remain limited.

Methods

Eimeria necatrix MIC3 gene (EnMIC3) was amplified and expressed in Escherichia coli. The recombinant protein (rEnMIC3) was characterized via SDS-PAGE and Western blot. The antigenicity of rEnMIC3 and its localization in sporozoites (SZ) and second-generation merozoites (MZ-2) of E. necatrix were determined by Western blot and indirect immunofluorescence analyses (IFAs). The dynamic expression of EnMIC3 across different developmental stages and its impact on sporozoite invasion of host cells were analyzed. The immune protection provided by rEnMIC3 was evaluated in chickens using weight gain, lesion scores, oocyst production, anticoccidial index (ACI), and antibody levels.

Results

The open reading frame of EnMIC3 was 798 bp, encoding a 265-amino acid protein with a predicted molecular weight of 28.50 kDa. EnMIC3 contained a signal peptide and a single epidermal growth factor (EGF)-like domain. The rEnMIC3 with an approximate molecular weight of 36 kDa could be specifically recognized by convalescent sera from chickens infected with E. necatrix. The molecular mass of the native protein was approximately 35 kDa, and it localizes to the apical region in SZ but exhibits a cytoplasmic distribution in MZ-2. EnMIC3 mRNA was expressed at significantly higher levels in SZ than in MZ-2, whereas protein expression displayed an inverse pattern. Anti-rEnMIC3 polyclonal antibodies inhibited sporozoite invasion of DF-1 cells in a dose-dependent manner. Vaccination with rEnMIC3 conferred effective protection against E. necatrix challenge, with the high-dose group (200 µg) achieving the highest ACI value (171.32) and markedly elevated serum antibody levels.

Conclusions

These findings not only offer a foundation for understanding the role of EnMIC3 protein in the host invasion of E. necatrix but also present a potential protective antigen of E. necatrix for the development of a subunit vaccine against avian coccidiosis.

Graphical Abstract