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Background <p>Since the first finding of <i>Ehrlichia chaffeensis</i> in the Argentinian marsh deer populations in 2018, we have conducted ongoing surveillance efforts to better understand the ecological and epidemiological dynamics of this zoonotic tick-borne pathogen in the region.</p> Methods <p>Over a period of 7 years (2018–2024) we analyzed blood and tissue samples from 40 marsh deer (<i>Blastocerus dichotomus</i>) and 13 parasitic <i>Amblyomma triste</i> ticks in the Paraná River Delta, Argentina.</p> Results <p>We identified <i>E. chaffeensis</i> DNA in two deer blood samples and in the salivary glands of three <i>A. triste</i> ticks parasitizing one of those deer. The approach used for <i>E. chaffeensis</i> detection in the tick sample ensured that the identified DNA came from an active tick infection, not residual host blood in the gut. After sequencing generic genes (<i>16S ribosomal RNA [rRNA], 23S rRNA</i> and <i>groEl</i>) and the specific <i>E. chaffeensis</i> variable-length polymerase chain reaction target (VLPT) molecular marker, we identified a genotype previously reported in Argentina.</p> Conclusions <p>This first-time contemporary detection of zoonotic <i>E. chaffeensis</i> in both a wild vertebrate host and its parasitic generalist ticks in a natural setting, provides direct evidence of a putative transmission cycle in Argentina, highlighting the need of implementing multidisciplinary surveillance systems.</p> Graphical Abstract <p></p>

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Molecular detection of Ehrlichia chaffeensis in marsh deer (Blastocerus dichotomus) and their parasitic Amblyomma triste ticks in Argentina suggests a local transmission cycle

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  • 发表日期：

    2026年1月2日

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  • Eliana Carolina Guillemi,
  • María Marcela Orozco,
  • Iara Figini,
  • Paula Blanco,
  • Marisa Diana Farber

摘要

Background

Since the first finding of Ehrlichia chaffeensis in the Argentinian marsh deer populations in 2018, we have conducted ongoing surveillance efforts to better understand the ecological and epidemiological dynamics of this zoonotic tick-borne pathogen in the region.

Methods

Over a period of 7 years (2018–2024) we analyzed blood and tissue samples from 40 marsh deer (Blastocerus dichotomus) and 13 parasitic Amblyomma triste ticks in the Paraná River Delta, Argentina.

Results

We identified E. chaffeensis DNA in two deer blood samples and in the salivary glands of three A. triste ticks parasitizing one of those deer. The approach used for E. chaffeensis detection in the tick sample ensured that the identified DNA came from an active tick infection, not residual host blood in the gut. After sequencing generic genes (16S ribosomal RNA [rRNA], 23S rRNA and groEl) and the specific E. chaffeensis variable-length polymerase chain reaction target (VLPT) molecular marker, we identified a genotype previously reported in Argentina.

Conclusions

This first-time contemporary detection of zoonotic E. chaffeensis in both a wild vertebrate host and its parasitic generalist ticks in a natural setting, provides direct evidence of a putative transmission cycle in Argentina, highlighting the need of implementing multidisciplinary surveillance systems.

Graphical Abstract