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Background <p><i>Ixodes fuscipes</i> is a tick species found in the Southern Cone of America and the only member of the <i>Ixodes ricinus</i> complex present in Uruguay. Members of this complex are particularly recognized as vectors of diseases affecting human health, such as babesiosis, caused by parasites of the genus <i>Babesia</i> (Apicomplexa: Piroplasmida). However, even though potential hosts of <i>I. fuscipes</i> in Uruguay (rodents, birds, and artiodactyls) are known carriers of <i>Babesia</i> species, the potential role of <i>I. fuscipes</i> as a vector of piroplasmids has not been studied. </p> Methods <p>In this study, questing <i>I. fuscipes</i> ticks were collected from five locations in Uruguay, and the presence of piroplasmid DNA was assessed using polymerase chain reaction (PCR) to amplify fragments of the small subunit ribosomal RNA (<i>18S</i> rRNA) and cytochrome c oxidase subunit 1 (<i>COI</i>) genes.</p> Results <p>A total of 953 ticks (larvae, nymphs, and adults) were collected; 14 samples (two larval pools and 12 nymphs) tested positive. Genetic analyses using <i>18S</i> rDNA and <i>COI</i> sequences revealed the presence of undescribed <i>Babesia</i> lineages, belonging to the <i>Babesia odocoilei</i> clade and others to the <i>Babesia microti</i> sensu stricto clade. </p> Conclusions <p>This work represents the first association of <i>Babesia</i> spp. with <i>I. fuscipes</i> and highlights the importance of this type of study to detect and mitigate the emergence of diseases associated with these arthropods. </p> Graphical Abstract <p></p>

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A survey for piroplasmids in questing Ixodes fuscipes ticks reveals undescribed Babesia lineages in Uruguay

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年6月18日

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  • Rodrigo Alvez,
  • María L. Félix,
  • Adriana Santodomingo,
  • Pablo Parodi,
  • Richard Thomas,
  • Sebastián Muñoz-Leal,
  • Luis Carvalho,
  • José M. Venzal

摘要

Background

Ixodes fuscipes is a tick species found in the Southern Cone of America and the only member of the Ixodes ricinus complex present in Uruguay. Members of this complex are particularly recognized as vectors of diseases affecting human health, such as babesiosis, caused by parasites of the genus Babesia (Apicomplexa: Piroplasmida). However, even though potential hosts of I. fuscipes in Uruguay (rodents, birds, and artiodactyls) are known carriers of Babesia species, the potential role of I. fuscipes as a vector of piroplasmids has not been studied.

Methods

In this study, questing I. fuscipes ticks were collected from five locations in Uruguay, and the presence of piroplasmid DNA was assessed using polymerase chain reaction (PCR) to amplify fragments of the small subunit ribosomal RNA (18S rRNA) and cytochrome c oxidase subunit 1 (COI) genes.

Results

A total of 953 ticks (larvae, nymphs, and adults) were collected; 14 samples (two larval pools and 12 nymphs) tested positive. Genetic analyses using 18S rDNA and COI sequences revealed the presence of undescribed Babesia lineages, belonging to the Babesia odocoilei clade and others to the Babesia microti sensu stricto clade.

Conclusions

This work represents the first association of Babesia spp. with I. fuscipes and highlights the importance of this type of study to detect and mitigate the emergence of diseases associated with these arthropods.

Graphical Abstract