Background <p><i>Trypanosoma</i> are protozoa parasites that infect animals and can cause economic losses in cattle production. <i>Trypanosoma</i> live in the blood and are transmitted by hematophagous insects, such as flies in the genus <i>Tabanus.</i> Using ecological niche models, we explored the current geography of six common <i>Tabanus</i> species in Brazil, which are considered vectors of <i>Trypanosoma vivax</i> and <i>Tr. evansi</i> in the Neotropics.</p> Methods <p>We used georeferenced data and biotic and abiotic variables integrated using a fundamental ecological niche modeling approach. Modeling results from six <i>Tabanus</i> species were used to identify risk areas of <i>Trypanosoma</i> transmission in Latin America accounting for area predicted, landscape conditions, and density of livestock. We performed Jaccard, Schoener, and Hellinger metrics to indicate the ecological niche similarities of pairs of <i>Tabanus</i> species to identify known and likely vectors overlapping in distribution across geographies.</p> Results <p>Our results revealed significant ecological niche similarities for two <i>Tabanus</i> species (<i>T. pungens</i> and <i>T. sorbillans</i>), whereas <i>T. triangulum</i> and <i>T. importunus</i> have low ecological similarity. Ecological niche models predicted risk of <i>Trypanosoma</i> transmission across Neotropical countries, with the highest risk in southern South America, Venezuela, and central Mexico.</p> Conclusions <p>More than 1.6 billion cattle and 38 million horses are under a threat category for infection risk. Furthermore, we identified specific areas and livestock populations at high risk of trypanosomiasis in Latin America. This study reveals the areas, landscapes, and populations at risk of <i>Trypanosoma</i> infections in livestock in the Americas.</p> Graphical Abstract <p></p>