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Background <p>To strengthen the national energy supply, there is an increasing demand for domestically generated aviation fuels. Bio-derived advanced aviation fuels offer the opportunity to meet this domestic need while presenting a unique opportunity to investigate the production of novel aviation fuels. Isoprenol, a chemical precursor to such novel fuels, has been shown to be a biologically producible compound in model organisms, but its bio-producibility needs to be further explored in organisms more compatible with industrial bioproduction.</p> Results <p>In this work, we evaluate isoprenol production using the promising bioproduction yeast, <i>Rhodosporidium toruloides</i>. First, we show successful isoprenol production using the IPP-bypass pathways most successful in laboratory strains of <i>E. coli</i> and <i>S. cerevisiae</i>. Next, we demonstrate that increased flux through the mevalonate pathway only modestly increases isoprenol titers. Using proteomics, we identified a potential bottleneck in production at the final step in the IPP-bypass pathway and explored alternative enzymes for this step. Finally, the top three strains of <i>R. toruloides</i> were evaluated in sorghum hydrolysates generated using cholinium lysinate. Through this work, 93.1&#xa0;mg/L of isoprenol was produced in mock medium and 27.3&#xa0;mg/L in sorghum hydrolysates.</p> Conclusion <p>Together these results lay the foundation for future work for the production of isoprenol from bioproduction crops.</p>

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Evaluating isoprenol production using the IPP-bypass pathway in the oleaginous yeast Rhodosporidium toruloides

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年3月3日

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  • Valentina E. Garcia,
  • Gina M. Geiselman,
  • Hee Jin Hwang,
  • Yan Chen,
  • Jennifer W. Gin,
  • Jack McFadden-Mitchell,
  • Veronica Montgomery,
  • Ramu Kakumanu,
  • Shubhasish Goswami,
  • Joshua McCauley,
  • Paul A. Adamczyk,
  • Edward E. K. Baidoo,
  • Christopher J. Petzold,
  • Blake A. Simmons,
  • Taek Soon Lee,
  • John M. Gladden

摘要

Background

To strengthen the national energy supply, there is an increasing demand for domestically generated aviation fuels. Bio-derived advanced aviation fuels offer the opportunity to meet this domestic need while presenting a unique opportunity to investigate the production of novel aviation fuels. Isoprenol, a chemical precursor to such novel fuels, has been shown to be a biologically producible compound in model organisms, but its bio-producibility needs to be further explored in organisms more compatible with industrial bioproduction.

Results

In this work, we evaluate isoprenol production using the promising bioproduction yeast, Rhodosporidium toruloides. First, we show successful isoprenol production using the IPP-bypass pathways most successful in laboratory strains of E. coli and S. cerevisiae. Next, we demonstrate that increased flux through the mevalonate pathway only modestly increases isoprenol titers. Using proteomics, we identified a potential bottleneck in production at the final step in the IPP-bypass pathway and explored alternative enzymes for this step. Finally, the top three strains of R. toruloides were evaluated in sorghum hydrolysates generated using cholinium lysinate. Through this work, 93.1 mg/L of isoprenol was produced in mock medium and 27.3 mg/L in sorghum hydrolysates.

Conclusion

Together these results lay the foundation for future work for the production of isoprenol from bioproduction crops.