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Background <p>The recalcitrance of lignin is a major bottleneck in the efficient conversion of lignocellulosic biomass to bioethanol. Genetic reduction of lignin content represents a key strategy to overcome this barrier. This study focuses on characterizing the <i>brown midrib2</i> (<i>bm2</i>) maize mutant to assess its potential for improving bioethanol production.</p> Results <p>Using a near-isogenic line (BC<sub>4</sub>F<sub>5</sub>) harboring the <i>bm2</i> mutation, an 8.01% reduction in acid-insoluble lignin content in stalks was observed, with no significant change in cellulose or hemicellulose. This lignin reduction led to a 25.17% increase in glucose release upon sulfuric acid pretreatment. Most importantly, the <i>bm2</i> mutant showed significantly higher lignocellulosic bioethanol yields: 3.05&#xa0;g/L Ethanol 1 from the pretreatment hydrolysate (fermentation via <i>Pichia stipites</i>) and 25.88&#xa0;g/L Ethanol 2 from the cellulose residue (fermentation via <i>Saccharomyces cerevisiae</i>), corresponding to 59.07% and 38.58% increases over the wild-type control, respectively.</p> Conclusions <p>Our results provide direct evidence that the <i>bm2</i> mutation enhances lignocellulosic ethanol production by reducing lignin content and improving saccharification efficiency. This work underscores the value of <i>bm2</i> in breeding specialized corn varieties for sustainable biofuel feedstock.</p>

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Boosting lignocellulosic bioethanol production: fermentation of corn stalk using a lignin-reduced brown midrib 2 maize mutant

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年11月26日

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  • Jinyue Wang,
  • Huachuan Tuo,
  • Peng Cheng,
  • Fangying Chen,
  • Yuxiao Li,
  • Mengyu Yuan,
  • Wenji Lin,
  • Xiaonan Bao,
  • Junjie Sun,
  • Yaowen Guo,
  • Ziwei Wang,
  • Ying Wang,
  • Haochuan Li,
  • Xiaohuan Mu,
  • Qin Zhang,
  • Yanwen Yu,
  • Mingyue Gou

摘要

Background

The recalcitrance of lignin is a major bottleneck in the efficient conversion of lignocellulosic biomass to bioethanol. Genetic reduction of lignin content represents a key strategy to overcome this barrier. This study focuses on characterizing the brown midrib2 (bm2) maize mutant to assess its potential for improving bioethanol production.

Results

Using a near-isogenic line (BC4F5) harboring the bm2 mutation, an 8.01% reduction in acid-insoluble lignin content in stalks was observed, with no significant change in cellulose or hemicellulose. This lignin reduction led to a 25.17% increase in glucose release upon sulfuric acid pretreatment. Most importantly, the bm2 mutant showed significantly higher lignocellulosic bioethanol yields: 3.05 g/L Ethanol 1 from the pretreatment hydrolysate (fermentation via Pichia stipites) and 25.88 g/L Ethanol 2 from the cellulose residue (fermentation via Saccharomyces cerevisiae), corresponding to 59.07% and 38.58% increases over the wild-type control, respectively.

Conclusions

Our results provide direct evidence that the bm2 mutation enhances lignocellulosic ethanol production by reducing lignin content and improving saccharification efficiency. This work underscores the value of bm2 in breeding specialized corn varieties for sustainable biofuel feedstock.