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<p>This study is the first to apply dilute acid pretreatment (DAP) under different severity conditions to poplar wood genetically modified for the <i>cinnamyl alcohol dehydrogenase</i> (<i>CAD1</i>) gene, which is involved in the lignin biosynthesis pathway. The carefully selected pretreatment conditions resulted in glucose yields that were 15 points higher for the <i>hpCAD</i> poplar line than for the wild-type (WT) wood after 48&#xa0;h of enzymatic hydrolysis. To explain this higher saccharification rate, the chemical, spectral and structural changes in WT and <i>hpCAD</i> wood were analyzed in relation to the severity of the pretreatment process. Although few differences were found at the chemical level, variations in autofluorescence and cell deformation were more significant: at high severity, the cells of <i>hpCAD</i> wood observed by nanotomography were more easily deformed, but their middle lamella was more resistant than those of WT wood. All these differences are possibly explained by changes in the molecular structure of lignin in <i>hpCAD</i> wood, leading to the formation of more hydrophobic shorter monomer chains with fewer lignin‒carbohydrate interactions.</p> Graphical Abstract <p></p>

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Cinnamyl alcohol dehydrogenase downregulation in poplar wood increases saccharification after dilute acid pretreatment: a key role for lignin revealed by a multimodal investigation

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年3月6日

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  • Julien du Pasquier,
  • Aya Zoghlami,
  • Youri Naudin,
  • Annabelle Déjardin,
  • Gilles Pilate,
  • Gabriel Paës,
  • Patrick Perré

摘要

This study is the first to apply dilute acid pretreatment (DAP) under different severity conditions to poplar wood genetically modified for the cinnamyl alcohol dehydrogenase (CAD1) gene, which is involved in the lignin biosynthesis pathway. The carefully selected pretreatment conditions resulted in glucose yields that were 15 points higher for the hpCAD poplar line than for the wild-type (WT) wood after 48 h of enzymatic hydrolysis. To explain this higher saccharification rate, the chemical, spectral and structural changes in WT and hpCAD wood were analyzed in relation to the severity of the pretreatment process. Although few differences were found at the chemical level, variations in autofluorescence and cell deformation were more significant: at high severity, the cells of hpCAD wood observed by nanotomography were more easily deformed, but their middle lamella was more resistant than those of WT wood. All these differences are possibly explained by changes in the molecular structure of lignin in hpCAD wood, leading to the formation of more hydrophobic shorter monomer chains with fewer lignin‒carbohydrate interactions.

Graphical Abstract