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<p>A new series of spiro[1,3,4]thiadiazole derivatives based on thioxoacetamides were synthesized, characterized, and evaluated for anti-arthritic potential. The structures of all compounds (<b>2a–12a</b>) were confirmed using IR, NMR, and elemental analysis.&#xa0;In vitro&#xa0;anti-arthritic activity was assessed via protein denaturation inhibition and RBC membrane stabilization assays. Compounds&#xa0;<b>9a</b>,&#xa0;<b>10a</b>,&#xa0;<b>6a</b>,&#xa0;<b>6b</b>,&#xa0;<b>6c</b>, and&#xa0;<b>6d</b>&#xa0;showed superior activity compared to the standard drug indomethacin, with&#xa0;<b>9a</b>&#xa0;being the most potent (IC₅₀ = 29.6&#xa0;µg/mL for protein denaturation). Molecular docking against COX-2 (PDB: 5IKT) revealed strong binding affinities, especially for&#xa0;<b>9a</b>&#xa0;(− 8.59&#xa0;kcal/mol),&#xa0;<b>10a</b>&#xa0;(− 8.45&#xa0;kcal/mol), and&#xa0;<b>6a</b>&#xa0;(− 8.05&#xa0;kcal/mol). DFT studies indicated favorable electronic properties comparable to indomethacin. These results highlight the promising anti-arthritic potential of the synthesized spirothiadiazole derivatives.</p> Graphical Abstract <p></p>

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Synthesis, characterization, molecular docking and in vitro anti-arthritic activity of some novel spiro [1,3,4] thiadiazole derivatives based on thioxoacetamides

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年3月8日

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  • Ahmed M. El-Saghier,
  • Asmaa Abdul-Baset,
  • Omar M. El-Hady,
  • Aly Abdou,
  • Amany M. Hamed,
  • Asmaa M. Kadry

摘要

A new series of spiro[1,3,4]thiadiazole derivatives based on thioxoacetamides were synthesized, characterized, and evaluated for anti-arthritic potential. The structures of all compounds (2a–12a) were confirmed using IR, NMR, and elemental analysis. In vitro anti-arthritic activity was assessed via protein denaturation inhibition and RBC membrane stabilization assays. Compounds 9a, 10a, 6a, 6b, 6c, and 6d showed superior activity compared to the standard drug indomethacin, with 9a being the most potent (IC₅₀ = 29.6 µg/mL for protein denaturation). Molecular docking against COX-2 (PDB: 5IKT) revealed strong binding affinities, especially for 9a (− 8.59 kcal/mol), 10a (− 8.45 kcal/mol), and 6a (− 8.05 kcal/mol). DFT studies indicated favorable electronic properties comparable to indomethacin. These results highlight the promising anti-arthritic potential of the synthesized spirothiadiazole derivatives.

Graphical Abstract