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<p>The current article describes facile, selective and highly sensitive spectrofluorimetric methodology for the determination of Mirabegron (MBG). The approach originated from the drug’s reaction with Erythrosine B in an acidic buffered media leading to the production of a binary complex. This interaction caused a significant quenching in the intensity of the fluorescence of Erythrosine B at an emission wavelength ( ʎ<sub>em</sub> ) of 550&#xa0;nm, following excitation ( ʎ<sub>ex</sub>) at 520&#xa0;nm. Key parameters of the procedure were optimized before constructing the calibration curve. A direct proportionality was obtained between the fluorescence quenching and MBG concentrations ranging from 0.05 to 1.2&#xa0;µg/mL. Both limits of detection (LOD) and quantitation (LOQ) have been calculated to be 0.01 and 0.04&#xa0;µg/mL, respectively. Implementing the demands set by the ICH, the developed technique was assessed to evaluate the level of accuracy, precision, and robustness. The suggested approach was employeds to determine the drug being studied in pharmaceutical tablets. Moreover, the suggested spectrofluorimetric method exhibited an elevated level of sensitivity, making it suited for accurately determining the mentioned drug in real plasma samples with outstanding recovery and a low relative standard deviation. The environmental sustainability of the proposed method was systematically evaluated through AGREE (Analytical Greenness Metric) and BAGI (Blue Applicability Grade Index) assessments, demonstrating superior eco-friendliness compared to conventional HPLC and electroanalytical techniques (AGREE score: 0.72 vs. 0.55–0.62). In addition to confirmation of the environmentally safety of the proposed approach, its blueness has been assessed using Blue Applicability Grade Index.</p>

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Eco-friendly spectrofluorimetric determination of mirabegron in human plasma and tablets: greenness and blueness assessment

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年10月29日

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  • Sayed M. Derayea,
  • Ebtehal F. Anwer,
  • Deena A. M. Nour El-Deen

摘要

The current article describes facile, selective and highly sensitive spectrofluorimetric methodology for the determination of Mirabegron (MBG). The approach originated from the drug’s reaction with Erythrosine B in an acidic buffered media leading to the production of a binary complex. This interaction caused a significant quenching in the intensity of the fluorescence of Erythrosine B at an emission wavelength ( ʎem ) of 550 nm, following excitation ( ʎex) at 520 nm. Key parameters of the procedure were optimized before constructing the calibration curve. A direct proportionality was obtained between the fluorescence quenching and MBG concentrations ranging from 0.05 to 1.2 µg/mL. Both limits of detection (LOD) and quantitation (LOQ) have been calculated to be 0.01 and 0.04 µg/mL, respectively. Implementing the demands set by the ICH, the developed technique was assessed to evaluate the level of accuracy, precision, and robustness. The suggested approach was employeds to determine the drug being studied in pharmaceutical tablets. Moreover, the suggested spectrofluorimetric method exhibited an elevated level of sensitivity, making it suited for accurately determining the mentioned drug in real plasma samples with outstanding recovery and a low relative standard deviation. The environmental sustainability of the proposed method was systematically evaluated through AGREE (Analytical Greenness Metric) and BAGI (Blue Applicability Grade Index) assessments, demonstrating superior eco-friendliness compared to conventional HPLC and electroanalytical techniques (AGREE score: 0.72 vs. 0.55–0.62). In addition to confirmation of the environmentally safety of the proposed approach, its blueness has been assessed using Blue Applicability Grade Index.