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<p>The increasing problem of bacterial resistance to antibiotics necessitates the exploration of innovative antimicrobial strategies. Phage therapy is a promising solution; however, naturally occurring phages targeting resistant pathogens remain limited. This study aimed to isolate and identify phages that lyse carbapenem-resistant <i>Pseudomonas aeruginosa</i> (CRPA) from sewage and investigate the synergistic antimicrobial effects of phage therapy combined with allicin. A novel phage, vB_PaeP_GZMU_A1002, was successfully isolated and characterized, revealing a double-stranded DNA genome of 63,770&#xa0;bp with no virulence or resistance genes, indicating its safety for application. This phage demonstrated good stability across various temperatures and pH levels, effectively inhibited the CRPA growth within 12&#xa0;h, and achieved a biofilm clearance rate of about 50%. Notably, the combination of phage and allicin significantly enhanced antimicrobial efficacy, reducing effective concentrations of both agents. The synergistic action was likely due to increased damage to bacterial cell membranes and reduced cell surface hydrophobicity. This research highlights the potential of phage therapy, particularly when combined with natural compounds like allicin, as a viable approach to combat antibiotic-resistant infections.</p>

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A novel synergistic action of phage vB_PaeP_GZMU_A1002 with allicin against carbapenem-resistant Pseudomonas aeruginosa

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年1月6日

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  • Yuan Fu,
  • Jiayi Liu,
  • Yinglin Yin,
  • Kailin Yuan,
  • Ziru Zhou,
  • Lei Xu,
  • Sifang Dou,
  • Baoqi Cai,
  • Zhihuang He,
  • Wanyu Wang,
  • Qiang Xiao,
  • Ping Chen

摘要

The increasing problem of bacterial resistance to antibiotics necessitates the exploration of innovative antimicrobial strategies. Phage therapy is a promising solution; however, naturally occurring phages targeting resistant pathogens remain limited. This study aimed to isolate and identify phages that lyse carbapenem-resistant Pseudomonas aeruginosa (CRPA) from sewage and investigate the synergistic antimicrobial effects of phage therapy combined with allicin. A novel phage, vB_PaeP_GZMU_A1002, was successfully isolated and characterized, revealing a double-stranded DNA genome of 63,770 bp with no virulence or resistance genes, indicating its safety for application. This phage demonstrated good stability across various temperatures and pH levels, effectively inhibited the CRPA growth within 12 h, and achieved a biofilm clearance rate of about 50%. Notably, the combination of phage and allicin significantly enhanced antimicrobial efficacy, reducing effective concentrations of both agents. The synergistic action was likely due to increased damage to bacterial cell membranes and reduced cell surface hydrophobicity. This research highlights the potential of phage therapy, particularly when combined with natural compounds like allicin, as a viable approach to combat antibiotic-resistant infections.