ScholarLink (思领科)
错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

IDMME and IDMDE: inducible CRISPR-dCasRx platforms for spatiotemporal RNA m5C editing

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年6月12日

查看全文
  • Jinming Xu,
  • Jiaju Xu,
  • Congcong Cao,
  • Yichang Shu,
  • Letian Shen,
  • Xuhong He,
  • Qi Huangfu,
  • Chengfang Sun,
  • Weikai Wang,
  • Jingchao Wei,
  • Ming Cai,
  • Bohan Wang,
  • Aolin Li,
  • Yuchen Liu,
  • Jiaming Wen

摘要

RNA 5-methylcytosine (m5C) is a dynamic epigenetic mark implicated in tumorigenesis, however, existing editors lack spatiotemporal regulation and reversibility. Here, we develop abscisic acid (ABA)-inducible CRISPR-dCasRx systems for programmable m5C methylation (IDMME) and demethylation (IDMDE). These editors enable site-specific, low off-target m5C modification through ligand-dependent assembly of split effector domains. We further integrate photocaged ABA to achieve light-controlled activation. Application in renal carcinoma models shows that targeted m5C editing modulates transcript function and suppresses tumor growth in vitro and in vivo. This platform provides a versatile, spatiotemporally controllable approach for dissecting RNA epigenetic mechanisms and advancing RNA-based therapeutic strategies.