Scholarlink

Background <p>Chromatin condensation, accompanied by pronounced nuclear shrinkage, is a pivotal step in terminal erythroid differentiation and is essential for enucleation. However, the precise timing and molecular mechanisms initiating this process remain poorly understood, particularly as global transcriptional repression occurs only at the orthochromatic erythroblast stage.</p> Results <p>Here we perform a comprehensive analysis of three-dimensional chromatin architecture dynamics during erythropoiesis using integrative epigenomic and imaging approaches. We demonstrate that chromatin condensation begins earlier than previously recognized, initiating at the late basophilic erythroblast stage. Mechanistically, these intergenic regions were tethered to the nuclear lamina (predominantly heterochromatic) and enriched in H3K9me3, which drive large-scale chromatin compaction. Furthermore, we find that the redistribution of H3K9me3 and dynamic remodeling of Lamin B1 are critical for this structural transition, directly impacting erythroid maturation.</p> Conclusions <p>These findings reveal a previously unrecognized regulatory axis linking H3K9me3 and chromatin-lamina interactions, providing novel insights into the spatial and temporal control of chromatin organization during erythroid development.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Dynamic changes in Lamin B1 and heterochromatin coincide with chromatin condensation during human erythropoiesis

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年3月24日

查看全文
  • Rui Wen,
  • Qiqi Zhang,
  • Yiqi Li,
  • Yijin Chen,
  • Yingru Jiang,
  • Yingjie Lu,
  • Junzhe Zhu,
  • Delin Kong,
  • Yulin Xu,
  • Xinhua Liu,
  • Pengxu Qian,
  • Meng Zhang,
  • He Huang,
  • Dawei Huo

摘要

Background

Chromatin condensation, accompanied by pronounced nuclear shrinkage, is a pivotal step in terminal erythroid differentiation and is essential for enucleation. However, the precise timing and molecular mechanisms initiating this process remain poorly understood, particularly as global transcriptional repression occurs only at the orthochromatic erythroblast stage.

Results

Here we perform a comprehensive analysis of three-dimensional chromatin architecture dynamics during erythropoiesis using integrative epigenomic and imaging approaches. We demonstrate that chromatin condensation begins earlier than previously recognized, initiating at the late basophilic erythroblast stage. Mechanistically, these intergenic regions were tethered to the nuclear lamina (predominantly heterochromatic) and enriched in H3K9me3, which drive large-scale chromatin compaction. Furthermore, we find that the redistribution of H3K9me3 and dynamic remodeling of Lamin B1 are critical for this structural transition, directly impacting erythroid maturation.

Conclusions

These findings reveal a previously unrecognized regulatory axis linking H3K9me3 and chromatin-lamina interactions, providing novel insights into the spatial and temporal control of chromatin organization during erythroid development.