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Background <p>Antimicrobial resistance in bacterial pathogens is a major threat to global health, rendering standard treatments ineffective and increasing the risk of severe infection or death. Resistance is often conferred by genes that are transferred horizontally among species and strains. However, for many bacteria, little is known about the genetic variation that potentiates resistance gene acquisition and accommodates acquired genes in the coadapted recipient genome.</p> Results <p>Here we introduce a new bioinformatics genome-wide association study approach, Guided Omission of Linkage Disequilibrium (GOLD-GWAS). This method masks covarying alleles explained by coinheritance and genome proximity to reveal loci where covarying sequence likely represents functional linkage, consistent with epistasis. Analysing 806 <i>Staphylococcus aureus</i> isolate genomes, including methicillin-resistant (MRSA) and methicillin-susceptible (MSSA) strains, we identified genes that covary with the presence of the acquired staphylococcal cassette chromosome <i>mec</i> (SCC<i>mec</i>) that houses the <i>mecA</i> resistance gene.</p> Conclusions <p>By uncovering known and new gene–gene associations, we demonstrate how resistance can involve genetic coalitions beyond well-known antimicrobial resistance genes. Understanding how genomic changes, such as extrinsic resistance cassettes, are integrated within coadapted bacterial genomes is an important step towards mitigating antimicrobial resistance evolution and identifying novel genetic targets for risk prediction, diagnosis, and therapy.</p>

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Genome co-adaptation and the evolution of methicillin resistant Staphylococcus aureus

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年2月11日

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  • Seungwon Ko,
  • Elizabeth A. Cummins,
  • William Monteith,
  • Samuel K. Sheppard

摘要

Background

Antimicrobial resistance in bacterial pathogens is a major threat to global health, rendering standard treatments ineffective and increasing the risk of severe infection or death. Resistance is often conferred by genes that are transferred horizontally among species and strains. However, for many bacteria, little is known about the genetic variation that potentiates resistance gene acquisition and accommodates acquired genes in the coadapted recipient genome.

Results

Here we introduce a new bioinformatics genome-wide association study approach, Guided Omission of Linkage Disequilibrium (GOLD-GWAS). This method masks covarying alleles explained by coinheritance and genome proximity to reveal loci where covarying sequence likely represents functional linkage, consistent with epistasis. Analysing 806 Staphylococcus aureus isolate genomes, including methicillin-resistant (MRSA) and methicillin-susceptible (MSSA) strains, we identified genes that covary with the presence of the acquired staphylococcal cassette chromosome mec (SCCmec) that houses the mecA resistance gene.

Conclusions

By uncovering known and new gene–gene associations, we demonstrate how resistance can involve genetic coalitions beyond well-known antimicrobial resistance genes. Understanding how genomic changes, such as extrinsic resistance cassettes, are integrated within coadapted bacterial genomes is an important step towards mitigating antimicrobial resistance evolution and identifying novel genetic targets for risk prediction, diagnosis, and therapy.