Scholarlink

<p>While Stereo-seq-FFPE captures spatial gene expression in formalin-fixed paraffin-embedded (FFPE) samples, it is limited to transcriptomic data. Here, we introduce ST-FFPE-mIF, a protocol that integrates eight-plex immunofluorescence staining with spatial transcriptomics in the same FFPE tissue section. Our approach extends Stereo-seq-FFPE to phenotypic analysis by enabling single-cell resolution through nuclear or membrane-based cell segmentation. We demonstrate how ST-FFPE-mIF supports robust spatial multi-omic profiling in cancer samples, offering a comprehensive view of molecular hierarchies from transcriptome to phenome.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

ST-FFPE-mIF: integrating spatial transcriptomics and multiplex immunofluorescence in formalin-fixed paraffin-embedded tissues using Stereo-seq

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年12月16日

查看全文
  • Xue Zhang,
  • Meng Zhang,
  • Yuan Xu,
  • Yangyang Song,
  • Xingfeng Yang,
  • Yanan Wu,
  • Xuelin Zhao,
  • Dongsheng Ran,
  • Xin Liu,
  • Huaqiang Huang,
  • Wenxiao Lei,
  • Hongyan Li,
  • Yongfen Zhang,
  • Feng Xi,
  • Guibo Li,
  • Xing Liu,
  • Luohao Xu,
  • Ao Chen,
  • Sha Liao,
  • Jiajun Zhang

摘要

While Stereo-seq-FFPE captures spatial gene expression in formalin-fixed paraffin-embedded (FFPE) samples, it is limited to transcriptomic data. Here, we introduce ST-FFPE-mIF, a protocol that integrates eight-plex immunofluorescence staining with spatial transcriptomics in the same FFPE tissue section. Our approach extends Stereo-seq-FFPE to phenotypic analysis by enabling single-cell resolution through nuclear or membrane-based cell segmentation. We demonstrate how ST-FFPE-mIF supports robust spatial multi-omic profiling in cancer samples, offering a comprehensive view of molecular hierarchies from transcriptome to phenome.