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Background <p>Lettuce is a globally important leafy vegetable that exhibits diverse horticultural types and strong population structure, which complicates genetic analyses. To address this challenge, we develop the first multi-parent, advanced generation inter-cross (MAGIC) population for lettuce using 16 diverse founder lines.</p> Results <p>Whole-genome sequencing of the 16 founder lines and 381 inbred progeny reveal minimal population structure, enabling informative genome-wide association studies (GWAS). GWAS of the lettuce MAGIC population identifies numerous loci associated with key agricultural traits, including 51 for flowering time, 11 for leaf color, and 5 for leaf shape. Notably, loss-of-function mutations in the <i>LsphyB</i> and <i>LsphyC</i> genes, encoding phytochromes B and C, dramatically delay flowering in lettuce, which is in striking contrast to many other plant species. This unexpected finding highlights the unique genetic architecture controlling flowering time in lettuce. The wild-type <i>LsTCP4</i> gene plays critical roles in leaf flatness and its expression level is negatively correlated with leaf curvature. Additionally, a novel zinc finger protein (<i>ZFP</i>) gene is required for the development of lobed leaves; a point mutation leads to its loss of function and consequently converted lobed leaves to non-lobed leaves, as exhibited by most lettuce cultivars.</p> Conclusions <p>The MAGIC population’s lack of structure and high mapping resolution enables the efficient dissection of complex traits. The identified loci and candidate genes provide significant genetic resources for improving agronomic performance and leaf quality in lettuce.</p>

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Dissecting the genetic architecture of key agronomic traits in lettuce using a MAGIC population

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年3月23日

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  • Hongyun Chen,
  • Jiongjiong Chen,
  • Ruifang Zhai,
  • Dean Lavelle,
  • Yue Jia,
  • Qiwei Tang,
  • Ting Zhu,
  • Menglu Wang,
  • Zedong Geng,
  • Jianzhong Zhu,
  • Hui Feng,
  • Junru An,
  • Jiansheng Liu,
  • Weibo Li,
  • Shenzhao Deng,
  • Wandi Wang,
  • Weiyi Zhang,
  • Xiaoyan Zhang,
  • Guangbao Luo,
  • Xin Wang,
  • Sunil Kumar Sahu,
  • Huan Liu,
  • Richard Michelmore,
  • Wanneng Yang,
  • Tong Wei,
  • Hanhui Kuang

摘要

Background

Lettuce is a globally important leafy vegetable that exhibits diverse horticultural types and strong population structure, which complicates genetic analyses. To address this challenge, we develop the first multi-parent, advanced generation inter-cross (MAGIC) population for lettuce using 16 diverse founder lines.

Results

Whole-genome sequencing of the 16 founder lines and 381 inbred progeny reveal minimal population structure, enabling informative genome-wide association studies (GWAS). GWAS of the lettuce MAGIC population identifies numerous loci associated with key agricultural traits, including 51 for flowering time, 11 for leaf color, and 5 for leaf shape. Notably, loss-of-function mutations in the LsphyB and LsphyC genes, encoding phytochromes B and C, dramatically delay flowering in lettuce, which is in striking contrast to many other plant species. This unexpected finding highlights the unique genetic architecture controlling flowering time in lettuce. The wild-type LsTCP4 gene plays critical roles in leaf flatness and its expression level is negatively correlated with leaf curvature. Additionally, a novel zinc finger protein (ZFP) gene is required for the development of lobed leaves; a point mutation leads to its loss of function and consequently converted lobed leaves to non-lobed leaves, as exhibited by most lettuce cultivars.

Conclusions

The MAGIC population’s lack of structure and high mapping resolution enables the efficient dissection of complex traits. The identified loci and candidate genes provide significant genetic resources for improving agronomic performance and leaf quality in lettuce.