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Background <p>Follicular atresia is a major determinant of ovarian failure in multiparous sows. Non-coding RNAs (ncRNAs) play an important role in the regulatory mechanisms controlling apoptosis within ovarian granulosa cells (GCs).</p> Methods <p>The circular structure of circVEGFA was validated by RNase R and actinomycin D treatments. The function of circVEGFA during apoptosis in GCs was investigated by si-RNA transfection. Furthermore, competitive binding of circVEGFA and TMX4 to miR-21-3p was confirmed by a dual-luciferase reporter gene assay and co-transfection with their inhibitors or siRNA. Results: In this study, we present a novel circular RNA (circRNA), circVEGFA, which shows significantly reduced expression in atretic follicles (AFs) compared to healthy follicles (HFs).</p> Conclusions <p>The study demonstrates that circVEGFA increases TMX4 expression and inhibits apoptosis in GCs through competitive binding to miR-21-3p. This study contributes to the understanding of circRNA regulation after follicular atresia.</p>

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circVEGFA inhibits apoptosis in porcine ovarian granulosa cells by binding to miR-21-3p and up-regulating TMX4 expression

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年7月17日

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  • Xinxin Qin,
  • Jinbi Zhang,
  • Chao Yin,
  • Fan Li,
  • Wenjie Li,
  • Xiaolong Cheng,
  • Xing Du,
  • Qifa Li,
  • Zengxiang Pan

摘要

Background

Follicular atresia is a major determinant of ovarian failure in multiparous sows. Non-coding RNAs (ncRNAs) play an important role in the regulatory mechanisms controlling apoptosis within ovarian granulosa cells (GCs).

Methods

The circular structure of circVEGFA was validated by RNase R and actinomycin D treatments. The function of circVEGFA during apoptosis in GCs was investigated by si-RNA transfection. Furthermore, competitive binding of circVEGFA and TMX4 to miR-21-3p was confirmed by a dual-luciferase reporter gene assay and co-transfection with their inhibitors or siRNA. Results: In this study, we present a novel circular RNA (circRNA), circVEGFA, which shows significantly reduced expression in atretic follicles (AFs) compared to healthy follicles (HFs).

Conclusions

The study demonstrates that circVEGFA increases TMX4 expression and inhibits apoptosis in GCs through competitive binding to miR-21-3p. This study contributes to the understanding of circRNA regulation after follicular atresia.