Scholarlink

<p>Microglia, the resident immune cells in the central nervous system, play important roles not only in immune response but also in neurogenesis, synaptogenesis, and neural circuit formation. Microglia also surveil the brain environment via elongation and retraction of their processes. Previously, we found that the purine salvage pathway is involved in the regulation of morphology and dynamics of the microglial cell line BV2. Here, we show that intraperitoneal administration of mycophenolate mofetil (MMF), an inosine monophosphate dehydrogenase (IMPDH) inhibitor, reduces microglial branching during postnatal development. Imaging mass spectrometry analysis revealed that MMF administration decreases guanosine nucleotides in the brain. Interestingly, despite the essential role of guanosine nucleotides in cellular proliferation, MMF administration did not significantly affect microglial proliferation. On the other hand, MMF administration attenuated the level of GTP-bound forms of RhoA and Rac1 small GTPases. Notably, MMF administration decreased the number of branches, while process length remained unaffected. Since microglial branching affects microglial complexity and diversity, our findings suggest that guanosine nucleotide production is essential for generating proper microglial diversity.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Mycophenolate mofetil reduces the branching of microglial processes

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年1月11日

查看全文
  • Rin-ichiro Teruya,
  • Kentaro Ueda,
  • Takumi Taketomi,
  • Takushi Yamamoto,
  • Naoki Yamashita,
  • Hana Konno,
  • Fuminori Tsuruta

摘要

Microglia, the resident immune cells in the central nervous system, play important roles not only in immune response but also in neurogenesis, synaptogenesis, and neural circuit formation. Microglia also surveil the brain environment via elongation and retraction of their processes. Previously, we found that the purine salvage pathway is involved in the regulation of morphology and dynamics of the microglial cell line BV2. Here, we show that intraperitoneal administration of mycophenolate mofetil (MMF), an inosine monophosphate dehydrogenase (IMPDH) inhibitor, reduces microglial branching during postnatal development. Imaging mass spectrometry analysis revealed that MMF administration decreases guanosine nucleotides in the brain. Interestingly, despite the essential role of guanosine nucleotides in cellular proliferation, MMF administration did not significantly affect microglial proliferation. On the other hand, MMF administration attenuated the level of GTP-bound forms of RhoA and Rac1 small GTPases. Notably, MMF administration decreased the number of branches, while process length remained unaffected. Since microglial branching affects microglial complexity and diversity, our findings suggest that guanosine nucleotide production is essential for generating proper microglial diversity.