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Background <p>Within lignocellulosic biomass, xylose is the second most abundant sugar after glucose. As a renewable and sustainable substrate, it is gaining attention as a feedstock for microbial bioprocesses. In this study, we demonstrated the co-production of polyhydroxybutyrate (PHB) and violacein from xylose. We initially confirmed the feasibility of co-production through genome-scale metabolic simulations, followed by optimization using a hybrid expression system that combines a conventional tac promoter and synthetic promoter-ribosome-binding site-terminator (semi-endo PRT) elements in dual plasmids. Additionally, we assessed the antimicrobial activity of violacein against type I methanotrophs.</p> Results <p>Recombinant <i>Escherichia coli</i> DH5α harboring a hybrid system produced 111.3 ± 19.7 and 0.88 ± 0.23&#xa0;mg/L of PHB and violacein, respectively, in M9 using xylose as the sole carbon source, without tryptophan supplementation. Using the synthetic PRT system, 528.9 ± 104&#xa0;mg/g dry cell weight (DCW) of PHB was obtained. Additionally, violacein inhibits the growth of <i>Methylomicrobium alcaliphilum</i> 20Z at 9&#xa0;µg/mL in nitrate mineral salt medium containing methanol as the sole carbon source.</p> Conclusions <p>The use of lignocellulose-derived sugars for co-production offers an environmentally sustainable bio-manufacturing approach that contributes to greenhouse gas mitigation and supports the transition toward a circular bioeconomy.</p>

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Hybrid metabolic engineering enables xylose-driven co-production of polyhydroxybutyrate and violacein in Escherichia coli

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年3月9日

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  • Khoi Nhat Pham,
  • Eun Yeol Lee

摘要

Background

Within lignocellulosic biomass, xylose is the second most abundant sugar after glucose. As a renewable and sustainable substrate, it is gaining attention as a feedstock for microbial bioprocesses. In this study, we demonstrated the co-production of polyhydroxybutyrate (PHB) and violacein from xylose. We initially confirmed the feasibility of co-production through genome-scale metabolic simulations, followed by optimization using a hybrid expression system that combines a conventional tac promoter and synthetic promoter-ribosome-binding site-terminator (semi-endo PRT) elements in dual plasmids. Additionally, we assessed the antimicrobial activity of violacein against type I methanotrophs.

Results

Recombinant Escherichia coli DH5α harboring a hybrid system produced 111.3 ± 19.7 and 0.88 ± 0.23 mg/L of PHB and violacein, respectively, in M9 using xylose as the sole carbon source, without tryptophan supplementation. Using the synthetic PRT system, 528.9 ± 104 mg/g dry cell weight (DCW) of PHB was obtained. Additionally, violacein inhibits the growth of Methylomicrobium alcaliphilum 20Z at 9 µg/mL in nitrate mineral salt medium containing methanol as the sole carbon source.

Conclusions

The use of lignocellulose-derived sugars for co-production offers an environmentally sustainable bio-manufacturing approach that contributes to greenhouse gas mitigation and supports the transition toward a circular bioeconomy.