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Background <p>For acute phase proteins to be implemented in routine diagnostics for pig herds, a fast, reliable, and portable point-of-care test is required. Therefore, this study aimed to evaluate the diagnostic performance of two point-of-care tests on lame gestating sows. The two tests were the Cobas b101 CRP test for C-reactive protein and OmniChek<sup>TM</sup>-SAA for serum amyloid A. The reference test was an enzyme-linked immunosorbent assay (ELISA).</p> Results <p>The C-reactive protein point-of-care test had a concordance with the ELISA results of 0.12 (95% confidence limits: 0.12–0.23) and a mean difference of − 30.98&#xa0;µg/mL (Limits of agreement: 27.45 and − 89.40&#xa0;µg/mL), with a clear proportional bias. The serum amyloid A point-of-care test was only in agreement with the ELISA results in two out of 49 samples. The serum amyloid A test overestimated the serum amyloid A levels, whereas the C-reactive protein point-of-care test underestimated the C-reactive protein levels.</p> Conclusions <p>Currently, the two point-of-care tests cannot be reliably used to determine acute phase protein levels in blood samples from lame gestating sows.</p>

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The diagnostic performance of point-of-care tests for serum amyloid A and C-reactive protein in lame sows

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年3月25日

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  • Nadia Jakobsen,
  • Inge Larsen,
  • Nicolai Rosager Weber,
  • Ken Steen Pedersen

摘要

Background

For acute phase proteins to be implemented in routine diagnostics for pig herds, a fast, reliable, and portable point-of-care test is required. Therefore, this study aimed to evaluate the diagnostic performance of two point-of-care tests on lame gestating sows. The two tests were the Cobas b101 CRP test for C-reactive protein and OmniChekTM-SAA for serum amyloid A. The reference test was an enzyme-linked immunosorbent assay (ELISA).

Results

The C-reactive protein point-of-care test had a concordance with the ELISA results of 0.12 (95% confidence limits: 0.12–0.23) and a mean difference of − 30.98 µg/mL (Limits of agreement: 27.45 and − 89.40 µg/mL), with a clear proportional bias. The serum amyloid A point-of-care test was only in agreement with the ELISA results in two out of 49 samples. The serum amyloid A test overestimated the serum amyloid A levels, whereas the C-reactive protein point-of-care test underestimated the C-reactive protein levels.

Conclusions

Currently, the two point-of-care tests cannot be reliably used to determine acute phase protein levels in blood samples from lame gestating sows.