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Background <p>Intervertebral disc degeneration is a leading cause of chronic low back pain, characterized by cellular senescence, extracellular matrix disintegration, and chronic inflammation. However, the key epigenetic regulators that orchestrate senescence and the resultant inflammatory cascade in disc degeneration remain poorly understood.</p> Objective <p>This study aimed to investigate the pathological role and molecular mechanism of HDAC9 in IDD.</p> Methods <p>We analyzed HDAC9 expression in aged degenerative discs. Using siRNA-mediated knockdown in primary cells, we assessed senescence, ECM, and inflammation. The mechanism was probed via co-immunoprecipitation, transcriptomics, and ELISA.</p> Results <p>HDAC9 expression was significantly upregulated in aged degenerative discs, correlating with enhanced cellular senescence and tissue destruction. Transcriptomic analysis revealed a profound pro-inflammatory and senescent signature in aged discs, with marked activation of the STAT1 signaling pathway. Knockdown of HDAC9 in aged cells attenuated senescence, reduced the expression of senescence markers and matrix catabolic enzyme, rescued proliferation deficits, and mitigated oxidative stress. Mechanistically, HDAC9 physically interacted with STAT1, and its knockdown decreased STAT1 phosphorylation. Both HDAC9 and STAT1 knockdown significantly suppressed the secretion of IL-6, TNF-α. Furthermore, classical STAT1 target genes were upregulated in aged discs.</p> Conclusion <p>Our findings demonstrate that HDAC9 is a critical epigenetic driver of age-related IDD. It promotes cellular senescence and a pro-inflammatory microenvironment by interacting with and activating the STAT1 signaling pathway. Targeting the HDAC9-STAT1 axis may represent a novel therapeutic strategy for mitigating disc degeneration.</p>

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HDAC9 drives intervertebral disc degeneration by potentiating STAT1-mediated senescence and inflammation

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年5月18日

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  • Xingdong Cheng,
  • Jingjing Wang,
  • Yuqing Chen,
  • Rong Xue,
  • Zengxin Gao

摘要

Background

Intervertebral disc degeneration is a leading cause of chronic low back pain, characterized by cellular senescence, extracellular matrix disintegration, and chronic inflammation. However, the key epigenetic regulators that orchestrate senescence and the resultant inflammatory cascade in disc degeneration remain poorly understood.

Objective

This study aimed to investigate the pathological role and molecular mechanism of HDAC9 in IDD.

Methods

We analyzed HDAC9 expression in aged degenerative discs. Using siRNA-mediated knockdown in primary cells, we assessed senescence, ECM, and inflammation. The mechanism was probed via co-immunoprecipitation, transcriptomics, and ELISA.

Results

HDAC9 expression was significantly upregulated in aged degenerative discs, correlating with enhanced cellular senescence and tissue destruction. Transcriptomic analysis revealed a profound pro-inflammatory and senescent signature in aged discs, with marked activation of the STAT1 signaling pathway. Knockdown of HDAC9 in aged cells attenuated senescence, reduced the expression of senescence markers and matrix catabolic enzyme, rescued proliferation deficits, and mitigated oxidative stress. Mechanistically, HDAC9 physically interacted with STAT1, and its knockdown decreased STAT1 phosphorylation. Both HDAC9 and STAT1 knockdown significantly suppressed the secretion of IL-6, TNF-α. Furthermore, classical STAT1 target genes were upregulated in aged discs.

Conclusion

Our findings demonstrate that HDAC9 is a critical epigenetic driver of age-related IDD. It promotes cellular senescence and a pro-inflammatory microenvironment by interacting with and activating the STAT1 signaling pathway. Targeting the HDAC9-STAT1 axis may represent a novel therapeutic strategy for mitigating disc degeneration.