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Objective <p>This study aims to investigate the expression characteristics, clinical diagnostic value of miR-1290 in osteoporotic fracture (OPF) patients, and its regulatory mechanism in osteoblast function.</p> Methods <p>289 subjects were divided into healthy control, OP, and OPF groups. Serum miR-1290 levels were detected by RT-qPCR, and its diagnostic efficacy was evaluated via ROC curve analysis. Functional experiments were conducted using hFOB1.19 cells to explore miR-1290’s expression pattern during osteogenic differentiation and its effects on cell proliferation and differentiation. Bioinformatics prediction, dual-luciferase reporter assay, and RIP assay were used to verify the target gene of miR-1290.</p> Results <p>Serum miR-1290 levels showed a gradient decrease from healthy controls to OP and OPF patients. It had favorable diagnostic efficacy for distinguishing OPF from OP patients. OPF patients’ postoperative miR-1290 levels gradually increased. miR-1290 was upregulated during osteogenic differentiation and promoted osteoblast proliferation and differentiation. KDM5A was identified as a direct target of miR-1290, and their expressions were negatively correlated. KDM5A overexpression reversed miR-1290’s enhancement of osteoblast proliferation and differentiation.</p> Conclusion <p>miR-1290 is downregulated in OP and OPF patients and promotes osteoblast function by targeting KDM5A, which highlights the role of the miR-1290/KDM5A axis in OPF and offers new insights for clinical diagnosis and targeted therapy.</p>

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miR-1290 serves as a potential biomarker for osteoporotic fracture and regulates osteoblast function by targeting KDM5A

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年3月4日

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  • Xiaoqin Xu,
  • Jia Huang,
  • Qi Chen,
  • Wen-Hua Lu,
  • Wei-Bing Chen,
  • Xiaojing Zhou

摘要

Objective

This study aims to investigate the expression characteristics, clinical diagnostic value of miR-1290 in osteoporotic fracture (OPF) patients, and its regulatory mechanism in osteoblast function.

Methods

289 subjects were divided into healthy control, OP, and OPF groups. Serum miR-1290 levels were detected by RT-qPCR, and its diagnostic efficacy was evaluated via ROC curve analysis. Functional experiments were conducted using hFOB1.19 cells to explore miR-1290’s expression pattern during osteogenic differentiation and its effects on cell proliferation and differentiation. Bioinformatics prediction, dual-luciferase reporter assay, and RIP assay were used to verify the target gene of miR-1290.

Results

Serum miR-1290 levels showed a gradient decrease from healthy controls to OP and OPF patients. It had favorable diagnostic efficacy for distinguishing OPF from OP patients. OPF patients’ postoperative miR-1290 levels gradually increased. miR-1290 was upregulated during osteogenic differentiation and promoted osteoblast proliferation and differentiation. KDM5A was identified as a direct target of miR-1290, and their expressions were negatively correlated. KDM5A overexpression reversed miR-1290’s enhancement of osteoblast proliferation and differentiation.

Conclusion

miR-1290 is downregulated in OP and OPF patients and promotes osteoblast function by targeting KDM5A, which highlights the role of the miR-1290/KDM5A axis in OPF and offers new insights for clinical diagnosis and targeted therapy.