Scholarlink

Background <p>Previous studies have shown that let-7c-5p is significantly expressed at a lower level in the serum of fracture patients, suggesting that it may play a role in bone repair. Therefore, this study aims to explore how let-7c-5p regulates osteogenic differentiation in tibial fractures.</p> Methods <p>A total of 80 patients with tibial fractures and 83 healthy individuals were included in this study. The level of let-7c-5p in the serum were detected by RT-qPCR. Osteoblast cell line (MC3T3-E1) were cultured in vitro to induce osteogenic differentiation. RT-qPCR was used to detect the expression of let-7c-5p and osteogenic differentiation markers. The activity of alkaline phosphatase (ALP) was determined using an ALP assay kit. Dual-luciferase reporter gene assay and RNA immunoprecipitation were used to verify the targeting relationship between let-7c-5p and CDK8.</p> Results <p>In the early stage of tibial fractures, the level of let-7c-5p in the patient’s serum was significantly lower than that of the control group, and as the healing processes progressed, its level gradually increased. In osteogenic induction, let-7c-5p, the activity of ALP, and the levels of osteogenic markers all increase. Increasing the level of let-7c-5p significantly enhanced the expression of osteogenic markers, while inhibiting its expression would weaken this effect. let-7c-5p directly targeted and negatively regulated the expression of cyclin-dependent kinase 8 (CDK8). Overexpression of CDK8 could reverse the osteogenic effect mediated by let-7c-5p.</p> Conclusion <p>During the healing process of tibial fractures, let-7c-5p promotes osteogenic differentiation by inhibiting CDK8, thereby accelerating fracture healing.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

let-7c-5p promotes fracture healing by downregulating CDK8

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年1月24日

查看全文
  • Wei Liu,
  • Jinxiang Zhang,
  • Xi Chen,
  • Bin Cheng,
  • Qiang Li,
  • Lin Li,
  • Hongbin Yang

摘要

Background

Previous studies have shown that let-7c-5p is significantly expressed at a lower level in the serum of fracture patients, suggesting that it may play a role in bone repair. Therefore, this study aims to explore how let-7c-5p regulates osteogenic differentiation in tibial fractures.

Methods

A total of 80 patients with tibial fractures and 83 healthy individuals were included in this study. The level of let-7c-5p in the serum were detected by RT-qPCR. Osteoblast cell line (MC3T3-E1) were cultured in vitro to induce osteogenic differentiation. RT-qPCR was used to detect the expression of let-7c-5p and osteogenic differentiation markers. The activity of alkaline phosphatase (ALP) was determined using an ALP assay kit. Dual-luciferase reporter gene assay and RNA immunoprecipitation were used to verify the targeting relationship between let-7c-5p and CDK8.

Results

In the early stage of tibial fractures, the level of let-7c-5p in the patient’s serum was significantly lower than that of the control group, and as the healing processes progressed, its level gradually increased. In osteogenic induction, let-7c-5p, the activity of ALP, and the levels of osteogenic markers all increase. Increasing the level of let-7c-5p significantly enhanced the expression of osteogenic markers, while inhibiting its expression would weaken this effect. let-7c-5p directly targeted and negatively regulated the expression of cyclin-dependent kinase 8 (CDK8). Overexpression of CDK8 could reverse the osteogenic effect mediated by let-7c-5p.

Conclusion

During the healing process of tibial fractures, let-7c-5p promotes osteogenic differentiation by inhibiting CDK8, thereby accelerating fracture healing.