Background <p>Pest control workers may be exposed to zoonotic pathogens during their work, yet data on the prevalence of zoonotic infections in this occupational group in Germany is scarce. This study investigated the seroprevalence of antibodies against four pathogens commonly transmitted by rodents and pigeons among pest control workers in Germany.</p> Methods <p>Blood samples of participants were analysed for antibodies against hantavirus, <i>Leptospira</i>, lymphocytic choriomeningitis virus (LCM virus) and <i>Chlamydia psittaci</i>. Additional data was collected using a questionnaire.</p> Results <p>Among the 154 participants, five were seropositive for hantavirus (3.2%), seven for <i>Leptospira</i> (4.6%) and two for <i>Chlamydia psittaci</i> (1.5%). No participants tested positive for LCM virus. Seropositive participants were mostly male (85.7%), aged 31–63 years and were predominantly engaged in field service pest control activities (85.7%). Both highly experienced (&gt; 20 years of experience) and fairly inexperienced (&lt; 1 year of experience) workers were affected. Notably, 42.9% of seropositive participants reported no or only occasional occupational exposure to the respective reservoir species. Most reported only occasional use of personal protective equipment (57.1%). Self-rated knowledge of zoonotic diseases was most commonly rated as moderate (50.0%) or good (42.9%).</p> Conclusion <p>Seroprevalence of antibodies against hantavirus, <i>Leptospira</i>, LCM virus, and <i>Chlamydia psittaci</i> was low among pest control workers in Germany. This may be indicative of effective infection control in this occupational group. Workers should receive intensified, regular training to further strengthen their knowledge of zoonotic diseases and improve adherence to precautionary measures.</p>