Evaluation of the FPMC respiratory panel for detection of respiratory tract pathogens in nasopharyngeal swab and sputum specimens

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2024年7月11日

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Li Xue,
Jianhong Zhu,
Ke Lei,
Zeshi Liu,
Yiwei Tang,
Bo Zhong,
Ning Gao,
Chaoliang Xiong,
Jing Lei,
Ying Tian,
Weixiao Zhou,
Nan Feng,
Xue Zhang,
Dong Chen,
Jing Li,
Yan Geng

摘要

Objectives

The performance of the new Respiratory Pathogen panel (fluorescent probe melting curve, FPMC) for the qualitative detection of 12 organisms (chlamydia pneumoniae, mycoplasma pneumoniae, adenovirus, influenza A virus, influenza B virus, parainfluenza virus, rhinovirus, etc.) was assessed.

Methods

Prospectively collected nasopharyngeal swab (NPS) and sputum specimens (n = 635) were detected by using the FPMC panel, with the Sanger sequencing method as the comparative method.

Results

The overall percent concordance between the FPMC analysis method and the Sanger sequencing method was 100% and 99.66% for NPS and sputum specimens, respectively. The FPMC testified an overall positive percent concordance of 100% for both NPS and sputum specimens. The FPMC analysis method also testified an overall negative percent concordance of 100% and 99.38% for NPS and sputum specimens, respectively.

Conclusions

The FPMC analysis method is a stable and accurate assay for rapid, comprehensive detecting for respiratory pathogens.