Differences in neutralizing antibody sensitivities and envelope characteristics indicate distinct antigenic properties of Nigerian HIV-1 subtype G and CRF02_AG
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发表日期:
2024年6月29日
- Lindsay Wieczorek,
- David Chang,
- Eric Sanders-Buell,
- Michelle Zemil,
- Elizabeth Martinez,
- Jesse Schoen,
- Agnes-Laurence Chenine,
- Sebastian Molnar,
- Brittani Barrows,
- Kultida Poltavee,
- Man E. Charurat,
- Alash’le Abimiku,
- William Blattner,
- Michael Iroezindu,
- Afoke Kokogho,
- Nelson L. Michael,
- Trevor A. Crowell,
- Julie A. Ake,
- Sodsai Tovanabutra,
- Victoria R. Polonis,
- Danielle Bartolanzo,
- Alexus Reynolds,
- Katherine Song,
- Mark Milazzo,
- Leilani Francisco,
- Shauna Mankiewicz,
- Steven Schech,
- Alexandra Golway,
- Badryah Omar,
- Tsedal Mebrahtu,
- Elizabeth Lee,
- Kimberly Bohince,
- Ajay Parikh,
- Jaclyn Hern,
- Emma Duff,
- Kara Lombardi,
- Michelle Imbach,
- Leigh Anne Eller,
- Hannah Kibuuka,
- Michael Semwogerere,
- Prossy Naluyima,
- Godfrey Zziwa,
- Allan Tindikahwa,
- Hilda Mutebe,
- Cate Kafeero,
- Enos Baghendaghe,
- William Lwebuge,
- Freddie Ssentogo,
- Hellen Birungi,
- Josephine Tegamanyi,
- Paul Wangiri,
- Christine Nabanoba,
- Phiona Namulondo,
- Richard Tumusiime,
- Ezra Musingye,
- Christina Nanteza,
- Joseph Wandege,
- Michael Waiswa,
- Evelyn Najjuma,
- Olive Maggaga,
- Isaac Kato Kenoly,
- Barbara Mukanza,
- Jonah Maswai,
- Rither Langat,
- Aaron Ngeno,
- Lucy Korir,
- Raphael Langat,
- Francis Opiyo,
- Alex Kasembeli,
- Christopher Ochieng,
- Japhet Towett,
- Jane Kimetto,
- Brighton Omondi,
- Mary Leelgo,
- Michael Obonyo,
- Linner Rotich,
- Enock Tonui,
- Ella Chelangat,
- Joan Kapkiai,
- Salome Wangare,
- Zeddy Bett Kesi,
- Janet Ngeno,
- Edwin Langat,
- Kennedy Labosso,
- Joshua Rotich,
- Leonard Cheruiyot,
- Enock Changwony,
- Mike Bii,
- Ezekiel Chumba,
- Susan Ontango,
- Danson Gitonga,
- Samuel Kiprotich,
- Bornes Ngtech,
- Grace Engoke,
- Irene Metet,
- Alice Airo,
- Ignatius Kiptoo,
- John Owuoth,
- Valentine Sing’oei,
- Winne Rehema,
- Solomon Otieno,
- Celine Ogari,
- Elkanah Modi,
- Oscar Adimo,
- Charles Okwaro,
- Christine Lando,
- Margaret Onyango,
- Iddah Aoko,
- Kennedy Obambo,
- Joseph Meyo,
- George Suja,
- Yakubu Adamu,
- Nnamdi Azuakola,
- Mfreke Asuquo,
- Abdulwasiu Bolaji Tiamiyu,
- Samirah Sani Mohammed,
- Ifeanyi Okoye,
- Sunday Odeyemi,
- Aminu Suleiman,
- Lawrence Umejo,
- Onome Enas,
- Miriam Mbachu,
- Ijeoma Chigbu-Ukaegbu,
- Wilson Adai,
- Felicia Anayochukwu Odo,
- Rabi Abdu,
- Rosemary Akiga,
- Helen Nwandu,
- CHisara Okolo,
- Ndubuisis Okeke,
- Zahra Parker,
- Asogwa Ugochukwu Linus,
- Concilia Amaka Agbaim,
- Tunde Adegbite,
- Nkenchiere Harrison,
- Adewale Adelakun,
- Ekeocha Chioma,
- Victoria Idi,
- Rachel Eluwa,
- Jumoke Nwalozie,
- Igiri Faith,
- Blessing Okanigbuan,
- Achugwo Emmanuel,
- Nkiru Nnadi,
- Ndubuisi Rosemary,
- Uzoegwu Amaka Natalie,
- Obende Theresa Owanza,
- Falaju Idowu Francis,
- Jacintal Elemere,
- Obilor Ifeoma Lauretta,
- Edward Akinwale,
- Inalegwu Ochai,
- Lucas Maganga,
- Emmanuel Bahemana,
- Samoel Khamadi,
- John Njegite,
- Connie Lueer,
- Abisai Kisinda,
- Jaquiline Mwamwaja,
- Faraja Mbwayu,
- Gloria David,
- Mtasi Mwaipopo,
- Reginald Gervas,
- Doroth Mkondoo,
- Nancy Somi,
- Paschal Kiliba,
- Gwamaka Mwaisanga,
- Johnisius Msigwa,
- Hawa Mfumbulwa,
- Peter Edwin,
- Willyhelmina Olomi,
- Manhattan Charurat,
- Aka Abayomi,
- Sylvia Adebajo,
- Stefan Baral,
- Charlotte Gaydos,
- Fengming Hu,
- Jennifer Malia,
- Rebecca Nowak,
- Uchenna Ononaku,
- Sheila Peel,
- Habib Ramadhani,
- Merlin Robb,
- Cristina Rodriguez-Hart,
- Elizabeth Shoyemi,
- Abdulwasiu Tiamiyu,
- Sandhya Vasan
摘要
The magnitude of the HIV-1 epidemic in Nigeria is second only to the subtype C epidemic in South Africa, yet the subtypes prevalent in Nigeria require further characterization. A panel of 50 subtype G and 18 CRF02_AG Nigerian HIV-1 pseudoviruses (PSV) was developed and envelope coreceptor usage, neutralization sensitivity and cross-clade reactivity were characterized. These PSV were neutralized by some antibodies targeting major neutralizing determinants, but potentially important differences were observed in specific sensitivities (eg. to sCD4, MPER and V2/V3 monoclonal antibodies), as well as in properties such as variable loop lengths, number of potential N-linked glycans and charge, demonstrating distinct antigenic characteristics of CRF02_AG and subtype G. There was preferential neutralization of the matched CRF/subtype when PSV from subtype G or CRF02_AG were tested using pooled plasma. These novel Nigerian PSV will be useful to study HIV-1 CRF- or subtype-specific humoral immune responses for subtype G and CRF02_AG.