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Differences in neutralizing antibody sensitivities and envelope characteristics indicate distinct antigenic properties of Nigerian HIV-1 subtype G and CRF02_AG

  • Lindsay Wieczorek,
  • David Chang,
  • Eric Sanders-Buell,
  • Michelle Zemil,
  • Elizabeth Martinez,
  • Jesse Schoen,
  • Agnes-Laurence Chenine,
  • Sebastian Molnar,
  • Brittani Barrows,
  • Kultida Poltavee,
  • Man E. Charurat,
  • Alash’le Abimiku,
  • William Blattner,
  • Michael Iroezindu,
  • Afoke Kokogho,
  • Nelson L. Michael,
  • Trevor A. Crowell,
  • Julie A. Ake,
  • Sodsai Tovanabutra,
  • Victoria R. Polonis,
  • Danielle Bartolanzo,
  • Alexus Reynolds,
  • Katherine Song,
  • Mark Milazzo,
  • Leilani Francisco,
  • Shauna Mankiewicz,
  • Steven Schech,
  • Alexandra Golway,
  • Badryah Omar,
  • Tsedal Mebrahtu,
  • Elizabeth Lee,
  • Kimberly Bohince,
  • Ajay Parikh,
  • Jaclyn Hern,
  • Emma Duff,
  • Kara Lombardi,
  • Michelle Imbach,
  • Leigh Anne Eller,
  • Hannah Kibuuka,
  • Michael Semwogerere,
  • Prossy Naluyima,
  • Godfrey Zziwa,
  • Allan Tindikahwa,
  • Hilda Mutebe,
  • Cate Kafeero,
  • Enos Baghendaghe,
  • William Lwebuge,
  • Freddie Ssentogo,
  • Hellen Birungi,
  • Josephine Tegamanyi,
  • Paul Wangiri,
  • Christine Nabanoba,
  • Phiona Namulondo,
  • Richard Tumusiime,
  • Ezra Musingye,
  • Christina Nanteza,
  • Joseph Wandege,
  • Michael Waiswa,
  • Evelyn Najjuma,
  • Olive Maggaga,
  • Isaac Kato Kenoly,
  • Barbara Mukanza,
  • Jonah Maswai,
  • Rither Langat,
  • Aaron Ngeno,
  • Lucy Korir,
  • Raphael Langat,
  • Francis Opiyo,
  • Alex Kasembeli,
  • Christopher Ochieng,
  • Japhet Towett,
  • Jane Kimetto,
  • Brighton Omondi,
  • Mary Leelgo,
  • Michael Obonyo,
  • Linner Rotich,
  • Enock Tonui,
  • Ella Chelangat,
  • Joan Kapkiai,
  • Salome Wangare,
  • Zeddy Bett Kesi,
  • Janet Ngeno,
  • Edwin Langat,
  • Kennedy Labosso,
  • Joshua Rotich,
  • Leonard Cheruiyot,
  • Enock Changwony,
  • Mike Bii,
  • Ezekiel Chumba,
  • Susan Ontango,
  • Danson Gitonga,
  • Samuel Kiprotich,
  • Bornes Ngtech,
  • Grace Engoke,
  • Irene Metet,
  • Alice Airo,
  • Ignatius Kiptoo,
  • John Owuoth,
  • Valentine Sing’oei,
  • Winne Rehema,
  • Solomon Otieno,
  • Celine Ogari,
  • Elkanah Modi,
  • Oscar Adimo,
  • Charles Okwaro,
  • Christine Lando,
  • Margaret Onyango,
  • Iddah Aoko,
  • Kennedy Obambo,
  • Joseph Meyo,
  • George Suja,
  • Yakubu Adamu,
  • Nnamdi Azuakola,
  • Mfreke Asuquo,
  • Abdulwasiu Bolaji Tiamiyu,
  • Samirah Sani Mohammed,
  • Ifeanyi Okoye,
  • Sunday Odeyemi,
  • Aminu Suleiman,
  • Lawrence Umejo,
  • Onome Enas,
  • Miriam Mbachu,
  • Ijeoma Chigbu-Ukaegbu,
  • Wilson Adai,
  • Felicia Anayochukwu Odo,
  • Rabi Abdu,
  • Rosemary Akiga,
  • Helen Nwandu,
  • CHisara Okolo,
  • Ndubuisis Okeke,
  • Zahra Parker,
  • Asogwa Ugochukwu Linus,
  • Concilia Amaka Agbaim,
  • Tunde Adegbite,
  • Nkenchiere Harrison,
  • Adewale Adelakun,
  • Ekeocha Chioma,
  • Victoria Idi,
  • Rachel Eluwa,
  • Jumoke Nwalozie,
  • Igiri Faith,
  • Blessing Okanigbuan,
  • Achugwo Emmanuel,
  • Nkiru Nnadi,
  • Ndubuisi Rosemary,
  • Uzoegwu Amaka Natalie,
  • Obende Theresa Owanza,
  • Falaju Idowu Francis,
  • Jacintal Elemere,
  • Obilor Ifeoma Lauretta,
  • Edward Akinwale,
  • Inalegwu Ochai,
  • Lucas Maganga,
  • Emmanuel Bahemana,
  • Samoel Khamadi,
  • John Njegite,
  • Connie Lueer,
  • Abisai Kisinda,
  • Jaquiline Mwamwaja,
  • Faraja Mbwayu,
  • Gloria David,
  • Mtasi Mwaipopo,
  • Reginald Gervas,
  • Doroth Mkondoo,
  • Nancy Somi,
  • Paschal Kiliba,
  • Gwamaka Mwaisanga,
  • Johnisius Msigwa,
  • Hawa Mfumbulwa,
  • Peter Edwin,
  • Willyhelmina Olomi,
  • Manhattan Charurat,
  • Aka Abayomi,
  • Sylvia Adebajo,
  • Stefan Baral,
  • Charlotte Gaydos,
  • Fengming Hu,
  • Jennifer Malia,
  • Rebecca Nowak,
  • Uchenna Ononaku,
  • Sheila Peel,
  • Habib Ramadhani,
  • Merlin Robb,
  • Cristina Rodriguez-Hart,
  • Elizabeth Shoyemi,
  • Abdulwasiu Tiamiyu,
  • Sandhya Vasan

摘要

The magnitude of the HIV-1 epidemic in Nigeria is second only to the subtype C epidemic in South Africa, yet the subtypes prevalent in Nigeria require further characterization. A panel of 50 subtype G and 18 CRF02_AG Nigerian HIV-1 pseudoviruses (PSV) was developed and envelope coreceptor usage, neutralization sensitivity and cross-clade reactivity were characterized. These PSV were neutralized by some antibodies targeting major neutralizing determinants, but potentially important differences were observed in specific sensitivities (eg. to sCD4, MPER and V2/V3 monoclonal antibodies), as well as in properties such as variable loop lengths, number of potential N-linked glycans and charge, demonstrating distinct antigenic characteristics of CRF02_AG and subtype G. There was preferential neutralization of the matched CRF/subtype when PSV from subtype G or CRF02_AG were tested using pooled plasma. These novel Nigerian PSV will be useful to study HIV-1 CRF- or subtype-specific humoral immune responses for subtype G and CRF02_AG.