<p>Food security and safety are essential pillars of the well-being and sustainability of any country. In Afghanistan, food safety challenges are particularly complex, arising from inadequate infrastructure, limited access to potable water, and widespread food insecurity. These conditions contribute to numerous health issues and pose significant risks to public health. These challenges underscore the urgent need for mandatory food safety training and certification programs to enhance hygiene practices among meat handlers in Tarinkot City. A cross-sectional study was conducted from May to July 2025, involving 70 meat handlers. Data were collected through face-to-face interviews using a structured questionnaire comprising three sections: sociodemographic characteristics, food safety knowledge, and food safety practices. The findings revealed that 85.7% of the participants demonstrated a high level of food safety awareness; however, 54.3% had inadequate knowledge of food poisoning, while 82.9% were aware of infectious diseases. The analysis also revealed significant associations between food safety knowledge and occupation, work experience, and possession of health certificates. This study highlights the need for targeted training programs aimed at improving personal and environmental hygiene and ensuring adherence to existing food safety regulations. It further recommends the development of comprehensive food safety policies within the One Health framework, supported by initiatives to raise awareness among all stakeholders involved in the food safety system.</p>