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Background <p>Psoriasis is a chronic, inflammatory autoimmune skin disease, characterized by epidermal hyperplasia and abnormal immune system activation. It is influenced by both genetic and environmental factors.</p> Methods <p>We identified hub genes associated with activated CD8 + T cell infiltration in psoriatic skin using machine learning algorithms. A LASSO regression model for psoriasis diagnosis was constructed based on these hub genes. Single-cell analysis identified a UBE2T+ Keratinocyte subpopulation, and spatial transcriptomics determined its location in the stratum spinosum. Additionally, in vitro experiments were conducted using a psoriatic keratinocyte cell model to assess the expression levels of UBE2T and its impact on cell proliferation and IL23A expression.</p> Results <p>Activated CD8 + T cells were significantly infiltrated in psoriatic skin. UBE2T was identified as a hub gene linked to activated CD8 + T cell infiltration. The UBE2T+ Keratinocyte subpopulation was located in the stratum spinosum of the epidermis, with the potential to differentiate into the UBE2T- Keratinocyte subpopulation. In vitro, UBE2T overexpression in HaCaT cells led to increased proliferation and upregulation of IL23A, a key pro-inflammatory factor.</p> Conclusions <p>UBE2T may serve as a potential diagnostic marker for psoriasis. The UBE2T+ Keratinocyte subpopulation is associated with excessive keratinocyte proliferation and inflammation, suggesting it could be a prospective target for psoriasis treatment.</p>

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Identification of UBE2T as a novel diagnostic biomarker of psoriasis and proliferating UBE2T+ Keratinocyte in psoriasis

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年2月11日

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  • Juntao Chen,
  • Li Peng,
  • Tengfei Yang,
  • Shuiqin Chen,
  • Ying Tang,
  • Dongfang Li,
  • Xiaoqing Yuan

摘要

Background

Psoriasis is a chronic, inflammatory autoimmune skin disease, characterized by epidermal hyperplasia and abnormal immune system activation. It is influenced by both genetic and environmental factors.

Methods

We identified hub genes associated with activated CD8 + T cell infiltration in psoriatic skin using machine learning algorithms. A LASSO regression model for psoriasis diagnosis was constructed based on these hub genes. Single-cell analysis identified a UBE2T+ Keratinocyte subpopulation, and spatial transcriptomics determined its location in the stratum spinosum. Additionally, in vitro experiments were conducted using a psoriatic keratinocyte cell model to assess the expression levels of UBE2T and its impact on cell proliferation and IL23A expression.

Results

Activated CD8 + T cells were significantly infiltrated in psoriatic skin. UBE2T was identified as a hub gene linked to activated CD8 + T cell infiltration. The UBE2T+ Keratinocyte subpopulation was located in the stratum spinosum of the epidermis, with the potential to differentiate into the UBE2T- Keratinocyte subpopulation. In vitro, UBE2T overexpression in HaCaT cells led to increased proliferation and upregulation of IL23A, a key pro-inflammatory factor.

Conclusions

UBE2T may serve as a potential diagnostic marker for psoriasis. The UBE2T+ Keratinocyte subpopulation is associated with excessive keratinocyte proliferation and inflammation, suggesting it could be a prospective target for psoriasis treatment.