Bulk RNA sequencing for analysis of post COVID-19 condition in adolescents and young adults

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2024年3月26日

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Silke Lauren Sommen,
Zhi Zhao,
Sunniva Segtnan,
Tonje Stiansen-Sonerud,
Joel Selvakumar,
Lise Beier Havdal,
Johannes Gjerstad,
Vegard Bruun Bratholm Wyller,
Lise Lund Berven

摘要

Background

Post COVID-19 condition (PCC) is a complication of SARS-COV-2 infection and can lead to long-term disability.

Methods

The present study was designed to analyse the gene expression patterns of PCC through bulk RNA sequencing of whole blood and to explore the potential molecular mechanisms of PCC. Whole blood was collected from 80 participants enrolled in a prospective cohort study following SARS-CoV-2 infected and non-infected individuals for 6 months after recruitment and was used for bulk RNA sequencing. Identification of differentially expressed genes (DEG), pathway enrichment and immune cell deconvolution was performed to explore potential biological pathways involved in PCC.

Results

We have found 13 differentially expressed genes associated with PCC. Enriched pathways were related to interferon-signalling and anti-viral immune processes.

Conclusion

The PCC transcriptome is characterized by a modest overexpression of interferon-stimulated genes, pointing to a subtle ongoing inflammatory response.