Scholarlink

<p>Organismal genome stability is constantly challenged by endogenous and exogenous genotoxins, and DNA damage response (DDR) is a classical cell-autonomous mechanism for counteracting such damage. It remains unclear how somatic cells with DDR deficiencies maintain genomic integrity under stress. This study investigated whether inter-tissue communication, particularly from the reproductive system, coordinates DNA damage repair in repair-deficient cells. Using <i>Caenorhabditis elegans</i>, in which certain upstream DDR signaling components are transcriptionally repressed in somatic cells, we examined UV-induced neurotoxicity through behavioral assays (head thrashes, body bends, foraging, and salt chemotaxis). These deficits were associated with preferential impairment of dopaminergic, glutamatergic, and serotonergic reporter readouts, along with heterogeneous transcriptional remodeling of neurotransmitter pathway and pan-neuronal synaptic genes. Deficiencies in upstream DDR signaling (ATM/ATR), whether systemic or germline-specific, significantly exacerbated UV-induced neurobehavioral and transcriptional deficits. In contrast, germline-specific loss of nucleotide excision repair (NER) machinery had no significant effect, highlighting a unique inter-tissue signaling role for upstream DDR. Furthermore, CPR-4 was identified as a key mediator of this inter gonad–soma signaling. Germline DDR activation regulated <i>cpr-4</i> expression, and loss of CPR-4 abolished UV-induced transcriptional activation of the somatic NER pathway and heightened behavioral sensitivity. Our study revealed a novel germline DDR to somatic tissue signaling axis essential for organismal stress resilience and proposed a new regulatory model centered on gonad–soma signaling.</p> Graphical abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Gonad–soma inter-tissue communication alleviates ultraviolet-induced neurotoxicity in C. elegans

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年7月23日

查看全文
  • Wenjing Zhang,
  • Liu Ding,
  • Shanpeng Qiao,
  • Jiyu Xu,
  • Qiyi Yi,
  • Binmei Liu,
  • Po Bian,
  • Ting Wang

摘要

Organismal genome stability is constantly challenged by endogenous and exogenous genotoxins, and DNA damage response (DDR) is a classical cell-autonomous mechanism for counteracting such damage. It remains unclear how somatic cells with DDR deficiencies maintain genomic integrity under stress. This study investigated whether inter-tissue communication, particularly from the reproductive system, coordinates DNA damage repair in repair-deficient cells. Using Caenorhabditis elegans, in which certain upstream DDR signaling components are transcriptionally repressed in somatic cells, we examined UV-induced neurotoxicity through behavioral assays (head thrashes, body bends, foraging, and salt chemotaxis). These deficits were associated with preferential impairment of dopaminergic, glutamatergic, and serotonergic reporter readouts, along with heterogeneous transcriptional remodeling of neurotransmitter pathway and pan-neuronal synaptic genes. Deficiencies in upstream DDR signaling (ATM/ATR), whether systemic or germline-specific, significantly exacerbated UV-induced neurobehavioral and transcriptional deficits. In contrast, germline-specific loss of nucleotide excision repair (NER) machinery had no significant effect, highlighting a unique inter-tissue signaling role for upstream DDR. Furthermore, CPR-4 was identified as a key mediator of this inter gonad–soma signaling. Germline DDR activation regulated cpr-4 expression, and loss of CPR-4 abolished UV-induced transcriptional activation of the somatic NER pathway and heightened behavioral sensitivity. Our study revealed a novel germline DDR to somatic tissue signaling axis essential for organismal stress resilience and proposed a new regulatory model centered on gonad–soma signaling.

Graphical abstract