Scholarlink

<p>It was recently shown that vertebrates, ranging from fish to primitive mammals, possess type IV IFN (IFNυ). However, their precise function and elaborate signaling remains unknown. In this study, <i>Ca</i>IFNυ was identified from <i>Carassius gibelio</i> and its transcriptional regulation and signaling was further investigated. Firstly, <i>Ca</i>IFNυ displays significant differences in the distribution, magnitude, and kinetics of its antiviral properties compared with <i>Ca</i>IFNa1, a typical type I IFN from <i>C. gibelio</i>. Secondly, IRF1/3/7 differentially activated <i>Ca</i>IFNυ through homo- or heteroprotein complexes in a dose-dependent fashion and NF-κB (p65) was the most effective stimulator. Thirdly, <i>Ca</i>IFNυ initiates the antiviral ISGs through the JAK-STAT as well as MAPK and PI3K signaling pathways by binding to the extra-cellular region of <i>Ca</i>CRFB4 and <i>Ca</i>CRFB12. Taken together, these findings reveal that <i>Ca</i>IFNυ belonges to an antiviral cytokine lineage which is distinct from <i>Ca</i>IFNa1.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Transcriptional regulation and signaling of type IV interferon in Carassius gibelio

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年7月11日

查看全文
  • Caijiao Dai,
  • Yuting Zhu,
  • Shiyu Qian,
  • Yihui Fan,
  • Zekun Weng,
  • Lijuan Li,
  • Jianghua Wang,
  • Junfa Yuan

摘要

It was recently shown that vertebrates, ranging from fish to primitive mammals, possess type IV IFN (IFNυ). However, their precise function and elaborate signaling remains unknown. In this study, CaIFNυ was identified from Carassius gibelio and its transcriptional regulation and signaling was further investigated. Firstly, CaIFNυ displays significant differences in the distribution, magnitude, and kinetics of its antiviral properties compared with CaIFNa1, a typical type I IFN from C. gibelio. Secondly, IRF1/3/7 differentially activated CaIFNυ through homo- or heteroprotein complexes in a dose-dependent fashion and NF-κB (p65) was the most effective stimulator. Thirdly, CaIFNυ initiates the antiviral ISGs through the JAK-STAT as well as MAPK and PI3K signaling pathways by binding to the extra-cellular region of CaCRFB4 and CaCRFB12. Taken together, these findings reveal that CaIFNυ belonges to an antiviral cytokine lineage which is distinct from CaIFNa1.