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Background <p>Previous studies have shown that miR-19a-3p is expressed at higher levels in melanoma tissues, suggesting that it may be involved in the progression of melanoma. This study sought to elucidate the biological function of miR-19a-3p in melanoma.</p> Methods <p>In this study, RT-qPCR was performed to measure the expression level of miR-19a-3p in melanoma tissues and cell lines. The relationship between miR-19a-3p and the prognosis of melanoma patients was assessed by Kaplan-Meier analysis and the Cox regression model. The effects of miR-19a-3p on cell proliferation, migration, and invasion were analyzed by Cell Counting Kit-8 (CCK-8) and Transwell assays. The targeting relationship between miR-19a-3p and adrenergic receptor β1 (ADRB1) was verified by Dual-luciferase reporter assay.</p> Results <p>miR-19a-3p was significantly overexpressed in melanoma tissues and cell lines. The high expression of miR-19a-3p was significantly associated with a higher TNM stage, lymph node metastasis, and decreased survival rate. Inhibition of miR-19a-3p could significantly weaken the proliferation, migration, and invasion abilities of melanoma cells. ADRB1 was a direct target of miR-19a-3p, and their expressions were negatively correlated. Knockdown of ADRB1 reversed the anti-tumor effect of miR-19a-3p inhibitor.</p> Conclusion <p>miR-19a-3p promoted the malignant progression of melanoma by targeting and inhibiting ADRB1. Its expression level can serve as an independent biomarker for evaluating the prognosis of melanoma patients.</p>

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miR-19a-3p accelerates the development of melanoma and reduces the prognosis of patients

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年2月4日

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  • Ting Kang,
  • Liwei Xu,
  • Gang Wang

摘要

Background

Previous studies have shown that miR-19a-3p is expressed at higher levels in melanoma tissues, suggesting that it may be involved in the progression of melanoma. This study sought to elucidate the biological function of miR-19a-3p in melanoma.

Methods

In this study, RT-qPCR was performed to measure the expression level of miR-19a-3p in melanoma tissues and cell lines. The relationship between miR-19a-3p and the prognosis of melanoma patients was assessed by Kaplan-Meier analysis and the Cox regression model. The effects of miR-19a-3p on cell proliferation, migration, and invasion were analyzed by Cell Counting Kit-8 (CCK-8) and Transwell assays. The targeting relationship between miR-19a-3p and adrenergic receptor β1 (ADRB1) was verified by Dual-luciferase reporter assay.

Results

miR-19a-3p was significantly overexpressed in melanoma tissues and cell lines. The high expression of miR-19a-3p was significantly associated with a higher TNM stage, lymph node metastasis, and decreased survival rate. Inhibition of miR-19a-3p could significantly weaken the proliferation, migration, and invasion abilities of melanoma cells. ADRB1 was a direct target of miR-19a-3p, and their expressions were negatively correlated. Knockdown of ADRB1 reversed the anti-tumor effect of miR-19a-3p inhibitor.

Conclusion

miR-19a-3p promoted the malignant progression of melanoma by targeting and inhibiting ADRB1. Its expression level can serve as an independent biomarker for evaluating the prognosis of melanoma patients.