<p>Wound healing is a complex process involving various factors and reconstruction of tissue structures. Platelets and their bioactive substances play an indispensable role in various stages of wound healing. Tissue engineering scaffolds are instrumental in maintaining, repairing, and enhancing tissue structure and function. Combining platelet concentrates with tissue engineering scaffolds heralds a novel direction for wound healing research. In this combination, platelet concentrates serve as tissue repair facilitators, while scaffolds function as tissue support and drug release platforms. This synergistic approach produces superior tissue repair outcomes in comparison to using either method independently. As a result, it is crucial to consolidate the application of platelet concentrates with tissue engineering scaffolds in the context of wound healing. This review begins by exploring the role of platelet concentrates and tissue engineering scaffolds in wound healing, while also outlining the essential criteria that scaffolds must meet. It then summarizes the application advances of platelet concentrates combined with tissue engineering scaffolds in wound healing. Finally, we highlighted current limitations in translation, including interpatient variability, formulation reproducibility, and complex regulatory hurdles. Developing innovative strategies and delving deeper into potential molecular mechanisms will drive further advancements in wound healing therapies through biomaterials engineering.</p> Graphical abstract <p></p>