Scholarlink

Background <p>The key to treating infectious bone defects (IBD) is regulating reactive oxygen species (ROS) balance to achieve “anti-infective first, then osteogenesis” temporal sequential repair. However, sensing microenvironmental changes to regulate ROS remains challenging. This study prepared a nanoparticle (PBD-ICG nanoparticlces) capable of sensing pH changes to enable protonation and deprotonation with “triple-element synergy” acid-base-driven assembly method, and loaded onto GelMA/HAMA hydrogel microsphere established a functional-responsiveness hydrogel microsphere (PGI) based on microenvironment sensing.</p> Result <p>With ultrasonic cavitation, indocyanine green transition to excited state, enabling electron transfer or energy transfer with H<sub>2</sub>O or <sup>3</sup>O₂ in microenvironment to generate ROS. Under 1&#xa0;W ultrasound, ROS release 74% to anti-bacteria and restores acidic infected microenvironment to normal weakly alkaline state. Simultaneously, nanoparticles sense pH changes, triggering internal polyaniline transform to emeraldine base and bovine serum albumin conformational changes. This process releases deferoxamine to inhibit excess ROS with 72% efficiency, regulating ROS balance and promoting vascularized osteogenesis. In vitro experiments confirmed a 9.19-fold increase in angiogenesis. In vivo studies revealed 82.6% antibacterial efficacy, followed by 46.5% improvement in osteogenesis efficacy, achieving temporal sequential treatment.</p> Conclusion <p>Summarizing, this system regulates ROS balance through sensing of microenvironment to achieve functional-responsive temporal sequential repair, providing novel strategies for IBD synergistic repair.</p> Graphical Abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Functional-responsive hydrogel microspheres based on microenvironment sensing promote the sequential repair of infected bone defects by regulating ROS balance

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年6月16日

查看全文
  • Xinyu Zhang,
  • Fan Wang,
  • Xinhe Li,
  • Xiaoqian Ding,
  • Anan Jang,
  • Zijie Wang,
  • Xu He,
  • Hui Gou,
  • Jindong Tan,
  • Wang Han,
  • Xiaoyu Han,
  • Dingqun Bai

摘要

Background

The key to treating infectious bone defects (IBD) is regulating reactive oxygen species (ROS) balance to achieve “anti-infective first, then osteogenesis” temporal sequential repair. However, sensing microenvironmental changes to regulate ROS remains challenging. This study prepared a nanoparticle (PBD-ICG nanoparticlces) capable of sensing pH changes to enable protonation and deprotonation with “triple-element synergy” acid-base-driven assembly method, and loaded onto GelMA/HAMA hydrogel microsphere established a functional-responsiveness hydrogel microsphere (PGI) based on microenvironment sensing.

Result

With ultrasonic cavitation, indocyanine green transition to excited state, enabling electron transfer or energy transfer with H2O or 3O₂ in microenvironment to generate ROS. Under 1 W ultrasound, ROS release 74% to anti-bacteria and restores acidic infected microenvironment to normal weakly alkaline state. Simultaneously, nanoparticles sense pH changes, triggering internal polyaniline transform to emeraldine base and bovine serum albumin conformational changes. This process releases deferoxamine to inhibit excess ROS with 72% efficiency, regulating ROS balance and promoting vascularized osteogenesis. In vitro experiments confirmed a 9.19-fold increase in angiogenesis. In vivo studies revealed 82.6% antibacterial efficacy, followed by 46.5% improvement in osteogenesis efficacy, achieving temporal sequential treatment.

Conclusion

Summarizing, this system regulates ROS balance through sensing of microenvironment to achieve functional-responsive temporal sequential repair, providing novel strategies for IBD synergistic repair.

Graphical Abstract