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<p>The precise spatial organization of neural cells into two-dimensional networks or three-dimensional spheroids is crucial for advancing neuroscience research and drug discoveries, yet remains challenging with conventional, single-function coatings. Here, we propose a programmable bifunctional peptide that integrates a silica-binding domain with a tunable cell-adhesive Arginine-Glycine-Aspartate (RGD) tripeptide. By systematically improving the RGD variant and linker rigidity, we introduced a single coating material that enables on-demand switching between two distinct functions: guiding the patterned growth of functional neural circuits on glass and facilitating the high-throughput formation of uniform neural spheroids. The latter exhibited high viability, extensive neurite outgrowth, and spontaneous electrophysiological activity, which validates their functional maturity. We establish by this work a versatile and reliable platform for advanced neural interface research, with significant potential for drug discovery and disease modeling.</p> Graphical abstract <p></p>

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A programmable peptide interface for on-demand neural culturing platforms

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年1月16日

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  • Hongyong Zhang,
  • Xixi Song,
  • Nan Huang,
  • Kang Xiong,
  • Nan Shao,
  • Yi Su,
  • Sumin Bian,
  • Mohamad Sawan

摘要

The precise spatial organization of neural cells into two-dimensional networks or three-dimensional spheroids is crucial for advancing neuroscience research and drug discoveries, yet remains challenging with conventional, single-function coatings. Here, we propose a programmable bifunctional peptide that integrates a silica-binding domain with a tunable cell-adhesive Arginine-Glycine-Aspartate (RGD) tripeptide. By systematically improving the RGD variant and linker rigidity, we introduced a single coating material that enables on-demand switching between two distinct functions: guiding the patterned growth of functional neural circuits on glass and facilitating the high-throughput formation of uniform neural spheroids. The latter exhibited high viability, extensive neurite outgrowth, and spontaneous electrophysiological activity, which validates their functional maturity. We establish by this work a versatile and reliable platform for advanced neural interface research, with significant potential for drug discovery and disease modeling.

Graphical abstract