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<p>During keratitis treatment, oxidative stress and inflammation often result in corneal neovascularisation, scarring, and reduced light transmittance. In this study, single-atom Pt/CeO<sub>2</sub> is synthesized, exhibiting significantly enhanced catalase-like and superoxide dismutase-like activities for the elimination of superoxide anions (•O<sub>2</sub><sup>−</sup>), hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>), and hydroxyl radicals (•OH). Doping single-atom Pt onto CeO<sub>2</sub> increases the Ce<sup>3+</sup> concentration in the Ce<sup>3+</sup>/Ce<sup>4+</sup> ratio from 39.12% to 58.66%, as confirmed by electron spin resonance, high-resolution transmission electron microscopy, X-ray photoelectron spectroscopy, and Raman spectroscopy. In vitro studies demonstrate that single-atom Pt/CeO<sub>2</sub> effectively reduces intracellular ROS levels in H<sub>2</sub>O<sub>2</sub>-activated human corneal epithelial cells. Additionally, it exerts an anti-inflammatory effect on LPS-stimulated RAW264.7 macrophages, significantly decreasing the expression of interleukin-1β, interleukin-6, and tumour necrosis factor-α. In vivo, in an LPS-induced keratitis animal model, single-atom Pt/CeO<sub>2</sub> accelerates corneal ulcer healing and preserves corneal light transmittance, attributed to its anti-inflammatory properties, enzyme-like activities, and ability to promote cell migration. This study offers a novel approach for treating various inflammatory and autoimmune diseases.</p> Graphical Abstract <p></p>

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Single-atom Pt doped nanoceria for enhanced cell phagocytosis and nanozyme activities in keratitis immune regulation

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年1月14日

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  • Jianguo Zhao,
  • Wanqing Lou,
  • Yixin Wang,
  • Lu Wang,
  • Xiaoqian Jin,
  • Chenyang Wang,
  • Jiangmeng Yao,
  • Jinjin Zhang,
  • Wenjuan Zhuang,
  • Jiayi Wei,
  • Dajun Lin,
  • Yishun Guo,
  • Yi Shao,
  • Bailiang Wang,
  • Zipei Jiang

摘要

During keratitis treatment, oxidative stress and inflammation often result in corneal neovascularisation, scarring, and reduced light transmittance. In this study, single-atom Pt/CeO2 is synthesized, exhibiting significantly enhanced catalase-like and superoxide dismutase-like activities for the elimination of superoxide anions (•O2−), hydrogen peroxide (H2O2), and hydroxyl radicals (•OH). Doping single-atom Pt onto CeO2 increases the Ce3+ concentration in the Ce3+/Ce4+ ratio from 39.12% to 58.66%, as confirmed by electron spin resonance, high-resolution transmission electron microscopy, X-ray photoelectron spectroscopy, and Raman spectroscopy. In vitro studies demonstrate that single-atom Pt/CeO2 effectively reduces intracellular ROS levels in H2O2-activated human corneal epithelial cells. Additionally, it exerts an anti-inflammatory effect on LPS-stimulated RAW264.7 macrophages, significantly decreasing the expression of interleukin-1β, interleukin-6, and tumour necrosis factor-α. In vivo, in an LPS-induced keratitis animal model, single-atom Pt/CeO2 accelerates corneal ulcer healing and preserves corneal light transmittance, attributed to its anti-inflammatory properties, enzyme-like activities, and ability to promote cell migration. This study offers a novel approach for treating various inflammatory and autoimmune diseases.

Graphical Abstract