Synergistic activity of gold nanoparticles with amphotericin B on persister cells of Candida tropicalis biofilms

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2024年5月16日

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M. A. Dasilva,
K. F. Crespo Andrada,
M. Maldonado Torales,
I. Manrrique Hughes,
P. Pez,
J. C. García-Martínez,
María Gabriela Paraje

摘要

Aim

The antifungal activity was studied on sessile and persister cells (PCs) of Candida tropicalis biofilms of gold nanoparticles (AuNPs) stabilized with cetyltrimethylammonium bromide (CTAB-AuNPs) and those conjugated with cysteine, in combination with Amphotericin B (AmB).

Materials/methods

The PC model was used and synergistic activity was tested by the checkerboard assay. Biofilms were studied by crystal violet and scanning electron microscopy.

Results/Conclusions

After the combination of both AuNPs and AmB the biofilm biomass was reduced, with significant differences in architecture being observed with a reduced biofilm matrix. In addition, the CTAB-AuNPs-AmB combination significantly reduced PCs. Understanding how these AuNPs aid in the fight against biofilms and the development of new approaches to eradicate PCs has relevance for chronic infection treatment.