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Aim <p>Located in the Southwest Indian Ocean area (SIOA), the two French overseas territories (FOTs) of Reunion and Mayotte islands are heavily impacted by antimicrobial resistance. The aim of this study was to investigate all cases of NDM-5 and OXA-181 carbapenemase-producing <i>Escherichia coli</i> (CP<i>Ec</i>) in these two FOTs between 2015 and 2020, to better understand the regional spread of these last-line treatment resistant bacteria.</p> Methods <p>All <i>E. coli</i> isolates not susceptible to ertapenem from various public and private hospitals on Reunion and Mayotte islands were screened for carbapenemase production. Clinical and microbiological data were collected for each case. Genotypic analysis of the isolates was carried out using WGS to determine the clonality relationship between the isolates and the genetic support of the carbapenemase-encoding genes.</p> Results <p>A total of 92 isolates of NDM-5 (<i>n</i> = 67) and OXA-181 (<i>n</i> = 25) CP<i>Ec</i> was collected from Reunion (<i>n</i> = 55) and Mayotte (<i>n</i> = 37) islands. Whole-genome sequencing identified 4 majors STs (ST58, ST167, ST405 and ST410). Genotypic analysis demonstrated numerous intra-ST possible cross transmission events, including strains isolated in both islands. Finally, all isolates (100%) carried the <i>bla</i><sub>NDM-5</sub> or <i>bla</i><sub>OXA-181</sub> genes on plasmids (IncF2, IncX3), most of which were conserved and identified in various STs.</p> Conclusion <p>We highlighted the dual dissemination of successful plasmids and the worrying circulation of high-risk clones <i>via </i>patients transfer between these two FOTs. It is therefore essential to effectively screen these patients for CP<i>Ec</i> carriage on admission and to take these plasmids into account when investigating intra- or inter-hospital CP<i>Ec</i> outbreaks.</p>

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Emergence and polyclonal dissemination of NDM-5/OXA-181 carbapenemase-producing Escherichia coli in the French Indian Ocean territories

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年1月29日

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  • Thibaut Vedani,
  • Matthieu Pot,
  • Thomas Garrigos,
  • Loïk Sababadichetty,
  • Marion Daniel,
  • David Wilkinson,
  • Thierry Benoit-Cattin,
  • Olivier Belmonte,
  • Patrick Mavingui,
  • Laurent Dortet,
  • Guillaume Miltgen

摘要

Aim

Located in the Southwest Indian Ocean area (SIOA), the two French overseas territories (FOTs) of Reunion and Mayotte islands are heavily impacted by antimicrobial resistance. The aim of this study was to investigate all cases of NDM-5 and OXA-181 carbapenemase-producing Escherichia coli (CPEc) in these two FOTs between 2015 and 2020, to better understand the regional spread of these last-line treatment resistant bacteria.

Methods

All E. coli isolates not susceptible to ertapenem from various public and private hospitals on Reunion and Mayotte islands were screened for carbapenemase production. Clinical and microbiological data were collected for each case. Genotypic analysis of the isolates was carried out using WGS to determine the clonality relationship between the isolates and the genetic support of the carbapenemase-encoding genes.

Results

A total of 92 isolates of NDM-5 (n = 67) and OXA-181 (n = 25) CPEc was collected from Reunion (n = 55) and Mayotte (n = 37) islands. Whole-genome sequencing identified 4 majors STs (ST58, ST167, ST405 and ST410). Genotypic analysis demonstrated numerous intra-ST possible cross transmission events, including strains isolated in both islands. Finally, all isolates (100%) carried the blaNDM-5 or blaOXA-181 genes on plasmids (IncF2, IncX3), most of which were conserved and identified in various STs.

Conclusion

We highlighted the dual dissemination of successful plasmids and the worrying circulation of high-risk clones via patients transfer between these two FOTs. It is therefore essential to effectively screen these patients for CPEc carriage on admission and to take these plasmids into account when investigating intra- or inter-hospital CPEc outbreaks.