Scholarlink

<p>Peripheral nerve injuries pose significant clinical challenges, leading to sensory and motor impairments, neuralgia, and socioeconomic burdens due to suboptimal recovery. This study evaluated the efficacy of electrospun polylactic acid (PLA) conduits combined with leupeptin and methylprednisolone for nerve regeneration in a rat sciatic nerve defect model. Fifty adult male Sprague–Dawley rats were randomized into five groups (<i>n</i> = 10/group): control, saline, leupeptin (0.5&#xa0;mg/mL), glucocorticoid (30&#xa0;mg/mL methylprednisolone), and combined (leupeptin + methylprednisolone). A 10-mm sciatic nerve gap was created and bridged with PLA conduits filled with the respective treatments. Functional recovery was assessed via the sciatic functional index (SFI) from weeks 4–16 postsurgery. Histological analyses (hematoxylin–eosin, Masson, and Ponceau red staining) and immunofluorescence for collagen I/III were performed at week 16. The combined group showed significantly higher SFI scores (e.g., − 20.5 ± 4.2 at week 16 vs. − 45.3 ± 5.1 in saline; <i>P</i> &lt; 0.001), enhanced regeneration, reduced fibrosis, and improved myelination compared to others. These results indicate that the combined approach optimizes the regenerative microenvironment, warranting further translational studies.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Synergistic enhancement of peripheral nerve regeneration using electrospun polylactic acid conduits with leupeptin and methylprednisolone in rats

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年4月13日

查看全文
  • Xiangchang Cao,
  • Fuzhou Yang,
  • Yaping Zhu,
  • Changqing Bai,
  • Jinwu Tang,
  • Jionghua Dai,
  • Luo Huang,
  • Qiming Chen

摘要

Peripheral nerve injuries pose significant clinical challenges, leading to sensory and motor impairments, neuralgia, and socioeconomic burdens due to suboptimal recovery. This study evaluated the efficacy of electrospun polylactic acid (PLA) conduits combined with leupeptin and methylprednisolone for nerve regeneration in a rat sciatic nerve defect model. Fifty adult male Sprague–Dawley rats were randomized into five groups (n = 10/group): control, saline, leupeptin (0.5 mg/mL), glucocorticoid (30 mg/mL methylprednisolone), and combined (leupeptin + methylprednisolone). A 10-mm sciatic nerve gap was created and bridged with PLA conduits filled with the respective treatments. Functional recovery was assessed via the sciatic functional index (SFI) from weeks 4–16 postsurgery. Histological analyses (hematoxylin–eosin, Masson, and Ponceau red staining) and immunofluorescence for collagen I/III were performed at week 16. The combined group showed significantly higher SFI scores (e.g., − 20.5 ± 4.2 at week 16 vs. − 45.3 ± 5.1 in saline; P < 0.001), enhanced regeneration, reduced fibrosis, and improved myelination compared to others. These results indicate that the combined approach optimizes the regenerative microenvironment, warranting further translational studies.