Background <p><i>Anopheles pseudopunctipennis</i> is the primary malaria vector in Mexico and is widely distributed nationwide. Understanding its developmental biology is crucial for interrupting malaria transmission.</p> Methods <p>This study was conducted in Tapachula, Chiapas, a city located in southeastern Mexico. Oviposition and fecundity patterns were determined in nulliparous and parous females, individually and in groups, under insectary and semi-field conditions.</p> Results <p>Regardless of whether they were grouped or individual, insectary-reared or field-collected, mosquito females exhibited a primary oviposition peak between 18:00 and 20:00, followed by a gradual decline until midnight. While nulliparous females from the insectary ceased oviposition after 24:00, a small percentage of parous females continued laying eggs throughout the night, until shortly before dawn (between 24:00 and 06:00). Parous females laid more eggs than nulliparous females</p> Conclusion <p>Nulliparous and parous females of <i>An. pseudopunctipennis</i> exhibited a major oviposition peak between 18:00 and 20:00, a few minutes after sunset. Oviposition activity persisted, albeit at a lower rate, until 24:00. A small subset of parous females continued ovipositing until 6:00. Parous females were observed laying more eggs than nulliparous females. Based on the high activity of <i>An. pseudopunctipennis</i>, from dusk until midnight, chemical control with pyrethroid insecticides applied by ultra-low volume (ULV) or by thermal fogging is proposed in breeding sites during this period, in areas near localities with active malaria transmission.</p>