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Background <p>Anaemia is a deleterious consequence of malaria, and its accurate diagnosis is crucial for effective management. However, laboratory methods for measuring haemoglobin (Hb) concentration, like the Coulter Counter and the Quantitative Buffy Coat® (QBC®), are costly and not widely accessible in resource-limited settings. The point-of-care HemoCue® test is a cheaper alternative and suitable in rural areas. The study aimed to determine the level of agreement between Coulter Counter/QBC® vs. HemoCue®-measured Hb concentrations by Bland–Altman analysis.</p> Methods <p>As part of a randomized, placebo-controlled trial of single low-dose primaquine in Ugandan and Congolese children with acute uncomplicated <i>Plasmodium falciparum</i> malaria, Hb concentrations were measured on days 0, 3, 7, and 28 using Coulter Counter (Uganda, n = 1880 paired values), QBC® (DR Congo, n = 1984 paired values) and HemoCue® Hb-301™. The predefined clinically acceptable limits were set at ± 0.5&#xa0;g/dL.</p> Results <p>The Bland–Altman analysis showed that the HemoCue® minus Coulter Counter mean Hb difference was −&#xa0;0.15&#xa0;g/dL with lower and upper limits of agreement of −&#xa0;3.68&#xa0;g/dL and 3.39&#xa0;g/dL, respectively. Corresponding HemoCue® minus QBC® values were −&#xa0;0.23&#xa0;g/dL, −&#xa0;1.66&#xa0;g/dL and 1.22&#xa0;g/dL. Linear regression of Hb concentration differences vs. mean Hb concentrations showed negative correlations: r = −&#xa0;0.43 and r = −&#xa0;0.34 for HemoCue® vs. Coulter Counter and HemoCue® vs. QBC®, respectively.</p> Conclusions <p>Compared to Coulter and QBC®, mean HemoCue® measured Hb concentrations were lower and, compared to the Coulter or QBC® methods, had an overall tendency to measure lower Hb concentrations with increasing Hb concentrations. Upper and lower limits of agreement were wider than the predefined clinically acceptable limits of ± 0.5&#xa0;g/dL. HemoCue® should be used with caution in settings where decisions about blood transfusions are made.</p>

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Comparing HemoCue® and Quantitative Buffy Coat® and Coulter Counter-measured haemoglobin concentrations in African children with acute uncomplicated malaria: a Bland–Altman analysis

  • 期刊论文

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  • 发表日期：

    2025年3月11日

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  • Dhol S. Ayuen,
  • Peter Olupot-Olupot,
  • Rita Muhindo,
  • Marie A. Onyamboko,
  • Seun Ajayi,
  • Natenapa Chimjinda,
  • Chiraporn Taya,
  • Sophie Uyoga,
  • Thomas N. Williams,
  • Kathryn Maitland,
  • Caterina Fanello,
  • Nicholas P. J. Day,
  • Walter R. Taylor,
  • Mavuto Mukaka

摘要

Background

Anaemia is a deleterious consequence of malaria, and its accurate diagnosis is crucial for effective management. However, laboratory methods for measuring haemoglobin (Hb) concentration, like the Coulter Counter and the Quantitative Buffy Coat® (QBC®), are costly and not widely accessible in resource-limited settings. The point-of-care HemoCue® test is a cheaper alternative and suitable in rural areas. The study aimed to determine the level of agreement between Coulter Counter/QBC® vs. HemoCue®-measured Hb concentrations by Bland–Altman analysis.

Methods

As part of a randomized, placebo-controlled trial of single low-dose primaquine in Ugandan and Congolese children with acute uncomplicated Plasmodium falciparum malaria, Hb concentrations were measured on days 0, 3, 7, and 28 using Coulter Counter (Uganda, n = 1880 paired values), QBC® (DR Congo, n = 1984 paired values) and HemoCue® Hb-301™. The predefined clinically acceptable limits were set at ± 0.5 g/dL.

Results

The Bland–Altman analysis showed that the HemoCue® minus Coulter Counter mean Hb difference was − 0.15 g/dL with lower and upper limits of agreement of − 3.68 g/dL and 3.39 g/dL, respectively. Corresponding HemoCue® minus QBC® values were − 0.23 g/dL, − 1.66 g/dL and 1.22 g/dL. Linear regression of Hb concentration differences vs. mean Hb concentrations showed negative correlations: r = − 0.43 and r = − 0.34 for HemoCue® vs. Coulter Counter and HemoCue® vs. QBC®, respectively.

Conclusions

Compared to Coulter and QBC®, mean HemoCue® measured Hb concentrations were lower and, compared to the Coulter or QBC® methods, had an overall tendency to measure lower Hb concentrations with increasing Hb concentrations. Upper and lower limits of agreement were wider than the predefined clinically acceptable limits of ± 0.5 g/dL. HemoCue® should be used with caution in settings where decisions about blood transfusions are made.