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Background &amp; aims <p>Hepatocellular carcinoma (HCC) is among the most common malignant tumors globally, characterized by high morbidity and mortality. This study aims to investigate the clinical and pathological significance of Apaf-1-interacting protein (<i>APIP)</i> expression in HCC, assess its biological effects on HCC cells, and explore the underlying mechanisms.</p> Methods <p>Internal tissue samples were analyzed to determine <i>APIP</i> expression and its clinical relevance in HCC using real-time quantitative polymerase chain reaction (RT-qPCR) and immunohistochemistry (IHC). External datasets were employed for verification. Experiments investigating cell proliferation, cell cycle progression, apoptosis, migration, and invasion were performed to understand the biological role and molecular mechanisms of <i>APIP</i> in HCC cells. Additionally, upstream transcription factors regulating <i>APIP</i> expression were explored.</p> Results <p><i>APIP</i> expressionwas significantly higher in HCC tissues compared to adjacent non-tumor tissues, effectively distinguishing HCC from normal liver tissues. <i>APIP</i> expression correlated with age, gender, and poor prognosis in patients with HCC. Furthermore, downregulation of <i>APIP</i> promoted apoptosis and inhibited proliferation, migration, and invasion of HCC cells. A regulatory relationship between <i>APIP</i> and the transcription factor Serum Response Factor (SRF) was also identified.</p> Conclusion <p><i>APIP</i> significantly contributes to the initiation and progression of HCC by mediating cell proliferation, apoptosis, migration, and invasion. Therefore, <i>APIP</i> may serve as a potential diagnostic and prognostic biomarker for HCC.</p>

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Clinical pathological significance and biological functions of APIP in hepatocellular carcinoma

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年10月22日

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  • Cong Yu,
  • Jiang-Hua Huang,
  • Rui Zhang,
  • Kang-Lai Wei,
  • Zhen-Bo Feng

摘要

Background & aims

Hepatocellular carcinoma (HCC) is among the most common malignant tumors globally, characterized by high morbidity and mortality. This study aims to investigate the clinical and pathological significance of Apaf-1-interacting protein (APIP) expression in HCC, assess its biological effects on HCC cells, and explore the underlying mechanisms.

Methods

Internal tissue samples were analyzed to determine APIP expression and its clinical relevance in HCC using real-time quantitative polymerase chain reaction (RT-qPCR) and immunohistochemistry (IHC). External datasets were employed for verification. Experiments investigating cell proliferation, cell cycle progression, apoptosis, migration, and invasion were performed to understand the biological role and molecular mechanisms of APIP in HCC cells. Additionally, upstream transcription factors regulating APIP expression were explored.

Results

APIP expressionwas significantly higher in HCC tissues compared to adjacent non-tumor tissues, effectively distinguishing HCC from normal liver tissues. APIP expression correlated with age, gender, and poor prognosis in patients with HCC. Furthermore, downregulation of APIP promoted apoptosis and inhibited proliferation, migration, and invasion of HCC cells. A regulatory relationship between APIP and the transcription factor Serum Response Factor (SRF) was also identified.

Conclusion

APIP significantly contributes to the initiation and progression of HCC by mediating cell proliferation, apoptosis, migration, and invasion. Therefore, APIP may serve as a potential diagnostic and prognostic biomarker for HCC.